Obesity alters the collagen organization and mechanical properties of murine cartilage.

Autor: Collins AT; Department of Orthopaedic Surgery, Duke University School of Medicine, DUMC Box 3093, Durham, NC, 27710, USA., Hu G; Department of Orthopaedic Surgery, Duke University School of Medicine, DUMC Box 3093, Durham, NC, 27710, USA., Newman H; Department of Orthopaedic Surgery, Duke University School of Medicine, DUMC Box 3093, Durham, NC, 27710, USA., Reinsvold MH; Department of Orthopaedic Surgery, Duke University School of Medicine, DUMC Box 3093, Durham, NC, 27710, USA., Goldsmith MR; Department of Orthopaedic Surgery, Duke University School of Medicine, DUMC Box 3093, Durham, NC, 27710, USA., Twomey-Kozak JN; Department of Orthopaedic Surgery, Duke University School of Medicine, DUMC Box 3093, Durham, NC, 27710, USA., Leddy HA; Shared Materials Instrumentation Facility, Pratt School of Engineering, Duke University, Durham, NC, 27710, USA., Sharma D; Department of Orthopaedic Surgery, Duke University School of Medicine, DUMC Box 3093, Durham, NC, 27710, USA., Shen L; Department of Orthopaedic Surgery, Duke University School of Medicine, DUMC Box 3093, Durham, NC, 27710, USA., DeFrate LE; Department of Orthopaedic Surgery, Duke University School of Medicine, DUMC Box 3093, Durham, NC, 27710, USA. lou.defrate@duke.edu.; Department of Biomedical Engineering, Pratt School of Engineering, Duke University, Durham, NC, 27710, USA. lou.defrate@duke.edu.; Department of Mechanical Engineering and Materials Science, Pratt School of Engineering, Duke University, Durham, NC, 27710, USA. lou.defrate@duke.edu., Karner CM; Department of Orthopaedic Surgery, Duke University School of Medicine, DUMC Box 3093, Durham, NC, 27710, USA.; Department of Cell Biology, Duke University School of Medicine, Durham, NC, 27710, USA.; Charles and Jane Pak Center for Mineral Metabolism and Clinical Research, Department of Internal Medicine, University of Texas Southwestern Medical Center, Dallas, TX, 75390, USA.
Jazyk: angličtina
Zdroj: Scientific reports [Sci Rep] 2021 Jan 15; Vol. 11 (1), pp. 1626. Date of Electronic Publication: 2021 Jan 15.
DOI: 10.1038/s41598-020-80599-1
Abstrakt: Osteoarthritis is a debilitating disease characterized by cartilage degradation and altered cartilage mechanical properties. Furthermore, it is well established that obesity is a primary risk factor for osteoarthritis. The purpose of this study was to investigate the influence of obesity on the mechanical properties of murine knee cartilage. Two-month old wild type mice were fed either a normal diet or a high fat diet for 16 weeks. Atomic force microscopy-based nanoindentation was used to quantify the effective indentation modulus of medial femoral condyle cartilage. Osteoarthritis progression was graded using the OARSI system. Additionally, collagen organization was evaluated with picrosirius red staining imaged using polarized light microscopy. Significant differences between diet groups were assessed using t tests with p < 0.05. Following 16 weeks of a high fat diet, no significant differences in OARSI scoring were detected. However, we detected a significant difference in the effective indentation modulus between diet groups. The reduction in cartilage stiffness is likely the result of disrupted collagen organization in the superficial zone, as indicated by altered birefringence on polarized light microscopy. Collectively, these results suggest obesity is associated with changes in knee cartilage mechanical properties, which may be an early indicator of disease progression.
Databáze: MEDLINE
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