In-vivo wound healing activity of a novel composite sponge loaded with mucilage and lipoidal matter of Hibiscus species.

Autor: Bakr RO; Department of Pharmacognosy, Faculty of Pharmacy, October University for Modern Sciences and Arts (MSA), 11787, Giza, Egypt. Electronic address: romar@msa.eun.eg., Amer RI; Department of Pharmaceutics and Industrial Pharmacy, Faculty of Pharmacy, Al-Azhar University, Cairo, Egypt; Department of Pharmaceutics, Faculty of Pharmacy, October University for Modern Sciences and Arts (MSA), Giza, Egypt., Attia D; Department of Pharmaceutics and Pharmaceutical Technology, Faculty of Pharmacy, The British University in Egypt (BUE), Cairo, Egypt., Abdelhafez MM; Department of Pharmacology, Faculty of Pharmacy, October University for Modern Sciences and Arts (MSA), Giza, Egypt., Al-Mokaddem AK; Department of Pathology, Faculty of Veterinary Medicine, Cairo University, 12211 Giza, Egypt., El-Gendy AEG; Department of Medicinal and Aromatic Plants Research, National Research Center, Giza, 12622, Egypt., El-Fishawy AM; Department of Pharmacognosy, Faculty of Pharmacy, Cairo University, 11562 Giza, Egypt., Fayed MAA; Department of Pharmacognosy, Faculty of Pharmacy, University of Sadat City, 32897, Egypt., Gad SS; Department of Pharmacology, Faculty of Pharmacy, October University for Modern Sciences and Arts (MSA), Giza, Egypt.
Jazyk: angličtina
Zdroj: Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie [Biomed Pharmacother] 2021 Mar; Vol. 135, pp. 111225. Date of Electronic Publication: 2021 Feb 01.
DOI: 10.1016/j.biopha.2021.111225
Abstrakt: Many researches have been undergone to hasten the natural wound healing process. In this study, several Hibiscus species (leaves) were extracted with petroleum ether, methanol, and their mucilage was separated. All the tested species extracts were assessed for their viability percentage using the water-soluble tetrazolium. H.syriacus was the plant of choice to be incorporated in a new drug delivery system and evaluated for its wound healing activity. H.syriacus petroleum ether extract (PEE) showed a high percentage of palmitic and oleic acids while its mucilage demonstrated high glucosamine and galacturonic acid. It was selected to be formulated and pharmaceutically evaluated into three different composite sponges using chitosan in various ratios. Fourier-transformed infrared spectroscopy investigated the chemical interaction between the utilized sponges' ingredients. Morphological characteristics were evaluated using scanning electron microscopy. H.syriacus composite sponge of mucilage: chitosan (1:5) was loaded with three different concentrations of PEE. Medicated formulations were assessed in rat model of excision wound model. The wound healing ability was clearly proved by the clinical acceleration, histopathological examination, and modulation of correlated inflammatory parameters as tumor necrosis factor in addition to vascular endothelial growth factor suggesting a promising valuable candidate that supports the management of excision wounds using single-dose preparation.
(Copyright © 2021 The Author(s). Published by Elsevier Masson SAS.. All rights reserved.)
Databáze: MEDLINE
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