Autor: |
Yabalak E; Mersin University, Faculty of Arts and Science, Department of Chemistry, Çiftlikköy Campus, TR-33343, Mersin, Turkey.; Mersin University, Technical Sciences Vocational School, Department of Food Technology, TR-33343, Mersin, Turkey., Erdoğan Eliuz EA; Mersin University, Technical Sciences Vocational School, Department of Food Technology, TR-33343, Mersin, Turkey., Nazlı MD; İzmir Biomedicine and Genome Center, Dokuz Eylül University, Health Campus, İzmir, Turkey. |
Abstrakt: |
In the present study, we evaluated the antibacterial activity of Citrus reticulata ( C. reticulata ) essential oil-incorporated gelatin film solution against Escherichia coli ( E. coli ) and Staphylococcus aureus ( S. aureus ). The minimum inhibitory concentration (MIC) of C. reticulata essential oil ( Cr EO) on E. coli and S. aureus were found to be 10.1 and 9.1 mg mL -1 , respectively, using spectrophotometric microdilution technique. The antimicrobial effect of Cr EO alone and incorporated gelatin-based film solutions ( Cr EO-F) against these pathogens were determined by the disc diffusion method. While the inhibition zones of CrEO were 2.4 mm and 10.05 mm, Cr EO-F was reported as 1.5 and 7.8 mm against E. coli and S. aureus , respectively. These findings demonstrated that the Cr EO can compete with coating agents with antimicrobial additives and it can find a place in the application areas. Besides, the chemical composition of the Cr EO was determined by GC-MS. |