| Autor: |
Anselm V; Signatope GmbH, Reutlingen, Germany., Steinhilber A; Signatope GmbH, Reutlingen, Germany., Sommersdorf C; Signatope GmbH, Reutlingen, Germany., Poetz O; Signatope GmbH, Reutlingen, Germany. poetz@signatope.com. |
| Jazyk: |
angličtina |
| Zdroj: |
Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2021; Vol. 2261, pp. 277-289. |
| DOI: |
10.1007/978-1-0716-1186-9_17 |
| Abstrakt: |
Targeted protein quantification can be challenging in body fluids such as plasma with regard to sensitivity and selectivity. In this chapter, we present a protocol for the quantification of high mobility group box 1 protein (HMGB1) in plasma using an immunoaffinity liquid chromatography mass spectrometric assay (IA-LC-MSMS). The protocol provides detailed assay instructions involving sample proteolysis, peptide-targeted immunoprecipitation, and LC-MSMS-based read out. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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