Global View on the Cytokinin Regulatory System in Potato.

Autor:	Lomin SN; Timiryazev Institute of Plant Physiology, Russian Academy of Sciences, Moscow, Russia., Myakushina YA; Timiryazev Institute of Plant Physiology, Russian Academy of Sciences, Moscow, Russia., Kolachevskaya OO; Timiryazev Institute of Plant Physiology, Russian Academy of Sciences, Moscow, Russia., Getman IA; Timiryazev Institute of Plant Physiology, Russian Academy of Sciences, Moscow, Russia., Savelieva EM; Timiryazev Institute of Plant Physiology, Russian Academy of Sciences, Moscow, Russia., Arkhipov DV; Timiryazev Institute of Plant Physiology, Russian Academy of Sciences, Moscow, Russia., Deigraf SV; Timiryazev Institute of Plant Physiology, Russian Academy of Sciences, Moscow, Russia., Romanov GA; Timiryazev Institute of Plant Physiology, Russian Academy of Sciences, Moscow, Russia.
Jazyk:	angličtina
Zdroj:	Frontiers in plant science [Front Plant Sci] 2020 Dec 21; Vol. 11, pp. 613624. Date of Electronic Publication: 2020 Dec 21 (Print Publication: 2020).
DOI:	10.3389/fpls.2020.613624
Abstrakt:	Cytokinins (CKs) were earlier shown to promote potato tuberization. Our study aimed to identify and characterize CK-related genes which constitute CK regulatory system in the core potato ( Solanum tuberosum ) genome. For that, CK-related genes were retrieved from the sequenced genome of the S. tuberosum doubled monoploid (DM) Phureja group, classified and compared with Arabidopsis orthologs. Analysis of selected gene expression was performed with a transcriptome database for the S. tuberosum heterozygous diploid line RH89-039-16. Genes responsible for CK signaling, biosynthesis, transport, and metabolism were categorized in an organ-specific fashion. According to this database, CK receptors StHK2/3 predominate in leaves and flowers, StHK4 in roots. Among phosphotransmitters, StHP1a expression largely predominates. Surprisingly, two pseudo-phosphotransmitters intended to suppress CK effects are hardly expressed in studied organs. Among B-type RR genes, StRR1b , StRR11 , and StRR18a are actively expressed, with StRR1b expressing most uniformly in all organs and StRR11 exhibiting the highest expression in roots. By cluster analysis four types of prevailing CK-signaling chains were identified in (1) leaves and flowers, StHK2/3→ S t H P 1 a →StRR1b/+; (2) shoot apical meristems, stolons, and mature tubers, StHK2/4→ S t H P 1 a →StRR1b/+; (3) stems and young tubers, StHK2/4→ S t H P 1 a →StRR1b/11/18a; and (4) roots and tuber sprouts, StHK4→ S t H P 1 a →StRR11/18a. CK synthesis genes StIPT3 / 5 and StCYP735A are expressed mainly in roots followed by tuber sprouts, but rather weakly in stolons and tubers. By contrast, CK-activation genes StLOGs are active in stolons, and StLOG3b expression is even stolon-confined. Apparently, the main CK effects on tuber initiation are realized via activity of StLOG1/3a/3b/7c/8a genes in stolons. Current advances and future directions in potato research are discussed. Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. (Copyright © 2020 Lomin, Myakushina, Kolachevskaya, Getman, Savelieva, Arkhipov, Deigraf and Romanov.)
Databáze:	MEDLINE
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