Influence of gamma radiation on Amphotericin B incorporated in PVP hydrogel as an alternative treatment for cutaneous leishmaniosis.

Autor: Oliveira MJA; Nuclear and Energy Research Institute-IPEN-CNEN/SP, Av. Professor Lineu Prestes, 2242, - Cidade Universitária CEP 05508-000, São Paulo-SP, Brazil. Electronic address: mariajhho@gmail.com., Villegas GME; CONACyT-Centro de Investigación en Química Aplicada, Av. Alianza Sur 204 Parque de Innovación e Investigación Tecnológica, Apodaca, Nuevo León, 66629 México., Motta FD; Faculdades Oswaldo Cruz Rua Brigadeiro Galvão, n° 540 - Barra Funda CEP, 01151-000, São Paulo-SP, Brazil., Fabela-Sánchez O; CONACyT-Centro de Investigación en Química Aplicada, Blvd. Enrique Reyna 140, Col, San José de los Cerritos, 25294, Saltillo, Coahuila, México., Espinosa-Roa A; CONACyT-Centro de Investigación en Química Aplicada, Av. Alianza Sur 204 Parque de Innovación e Investigación Tecnológica, Apodaca, Nuevo León, 66629 México., Fotoran WL; Departamento de Parasitologia, Universidade de São Paulo, Av. Professor Lineu Prestes, Cidade Universitária, 05508-000, São Paulo-SP, Brazil., Peixoto JC; Departamento de Parasitologia, Universidade de São Paulo, Av. Professor Lineu Prestes, Cidade Universitária, 05508-000, São Paulo-SP, Brazil., Tano FT; Departamento de Parasitologia, Universidade de São Paulo, Av. Professor Lineu Prestes, Cidade Universitária, 05508-000, São Paulo-SP, Brazil., Lugão AB; Nuclear and Energy Research Institute-IPEN-CNEN/SP, Av. Professor Lineu Prestes, 2242, - Cidade Universitária CEP 05508-000, São Paulo-SP, Brazil., Vásquez PAS; Nuclear and Energy Research Institute-IPEN-CNEN/SP, Av. Professor Lineu Prestes, 2242, - Cidade Universitária CEP 05508-000, São Paulo-SP, Brazil.
Jazyk: angličtina
Zdroj: Acta tropica [Acta Trop] 2021 Mar; Vol. 215, pp. 105805. Date of Electronic Publication: 2020 Dec 30.
DOI: 10.1016/j.actatropica.2020.105805
Abstrakt: Amphotericin B (Amph-B) is an antifungal drug used intravenously for the treatment of leishmaniasis. Side-effects from Amph-B treatment can arise such as cardiac arrhythmia and renal dysfunctions, which will lead to discontinuation of treatment. Unfortunately, patients in endemic countries do not have access to alternative therapies. The objective of this study was to analyze the effects of Cobalt-60 gamma irradiation on crosslinking polymeric hydrogels (Hydg) and the incorporation of Amph-B into the gel as a controlled-release drug delivery alternative. Polyvinylpyrrolidone (PVP)/Amph-B solutions were irradiated with 15 kGy at 0 °C and 25 °C. The drug's stability was ascertained by UV-visible spectrometry, liquid chromatography/mass spectrometry and proton nuclear magnetic resonance. Irradiated Hydg/Amph-B achieved similar stability to the standard Amph-B solution and was enough to promote hydrogel crosslinking. In vitro trials were carried out to ensure Amph-B was still biologically active after irradiation. The results from flow cytometry and MTT assay show that Amph-B had an IC 50  = 16.7 nM. A combination of Hydg at 1.324 gmL -1 and Amph-B at 25.1 nM for 24 h lead to the greatest inhibition of L. amazonensis promastigotes, and could be used as an alternative treatment method for cutaneous leishmaniosis.
(Copyright © 2020 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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