Adult Renal Stem/Progenitor Cells Can Modulate T Regulatory Cells and Double Negative T Cells.

Autor: Curci C; Nephrology, Dialysis and Transplantation Unit, DETO, University of Bari 'Aldo Moro', 70124, Bari, Italy.; Department of Basic Medical Sciences, Neuroscience and Sense Organs, University of Bari 'Aldo Moro', 70124, Bari, Italy., Picerno A; Nephrology, Dialysis and Transplantation Unit, DETO, University of Bari 'Aldo Moro', 70124, Bari, Italy., Chaoul N; Allergology Unit, DETO, University of Bari 'Aldo Moro', 70124, Bari, Italy., Stasi A; Nephrology, Dialysis and Transplantation Unit, DETO, University of Bari 'Aldo Moro', 70124, Bari, Italy., De Palma G; Institutional BioBank, Experimental Oncology and Biobank Management Unit, IRCCS Istituto Tumori 'Giovanni Paolo II', 70124, Bari, Italy., Franzin R; Nephrology, Dialysis and Transplantation Unit, DETO, University of Bari 'Aldo Moro', 70124, Bari, Italy., Pontrelli P; Nephrology, Dialysis and Transplantation Unit, DETO, University of Bari 'Aldo Moro', 70124, Bari, Italy., Castellano G; Nephrology, Dialysis and Transplantation Unit, Department of Medical and Surgical Science, University of Foggia, 71122, Foggia, Italy., Pertosa GB; Nephrology, Dialysis and Transplantation Unit, DETO, University of Bari 'Aldo Moro', 70124, Bari, Italy., Macchia L; Allergology Unit, DETO, University of Bari 'Aldo Moro', 70124, Bari, Italy., Di Lorenzo VF; Urology Unit, IRCCS Istituto Tumori 'Giovanni Paolo II', 70124, Bari, Italy., Sabbà C; Urology Unit, IRCCS Istituto Tumori 'Giovanni Paolo II', 70124, Bari, Italy., Gallone A; Department of Basic Medical Sciences, Neuroscience and Sense Organs, University of Bari 'Aldo Moro', 70124, Bari, Italy., Gesualdo L; Nephrology, Dialysis and Transplantation Unit, DETO, University of Bari 'Aldo Moro', 70124, Bari, Italy., Sallustio F; Department of Interdisciplinary Medicine, University of Bari 'Aldo Moro', 70124, Bari, Italy.
Jazyk: angličtina
Zdroj: International journal of molecular sciences [Int J Mol Sci] 2020 Dec 29; Vol. 22 (1). Date of Electronic Publication: 2020 Dec 29.
DOI: 10.3390/ijms22010274
Abstrakt: Adult Renal Stem/Progenitor Cells (ARPCs) have been recently identified in the human kidney and several studies show their active role in kidney repair processes during acute or chronic injury. However, little is known about their immunomodulatory properties and their capacity to regulate specific T cell subpopulations. We co-cultured ARPCs activated by triggering Toll-Like Receptor 2 (TLR2) with human peripheral blood mononuclear cells for 5 days and 15 days and studied their immunomodulatory capacity on T cell subpopulations. We found that activated-ARPCs were able to decrease T cell proliferation but did not affect CD8 + and CD4 + T cells. Instead, Tregs and CD3 + CD4 - CD8 - double-negative (DN) T cells decreased after 5 days and increased after 15 days of co-culture. In addition, we found that PAI1, MCP1, GM-CSF, and CXCL1 were significantly expressed by TLR2-activated ARPCs alone and were up-regulated in T cells co-cultured with activated ARPCs. The exogenous cocktail of cytokines was able to reproduce the immunomodulatory effects of the co-culture with activated ARPCs. These data showed that ARPCs can regulate immune response by inducing Tregs and DN T cells cell modulation, which are involved in the balance between immune tolerance and autoimmunity.
Databáze: MEDLINE
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