β-lactamases (bla TEM , bla SHV , bla CTXM-1 , bla VEB , bla OXA-1 ) and class C β-lactamases gene frequency in Pseudomonas aeruginosa isolated from various clinical specimens in Khartoum State, Sudan: a cross sectional study.
Autor: | Abdelrahman DN; Department of Virology, Central Laboratory, Khartoum, Sudan.; Department of Microbiology, College of Medical Laboratory Sciences, Sudan University of Science and Technology, Khartoum, Sudan., Taha AA; Department of Microbiology, College of Medical Laboratory Sciences, Sudan University of Science and Technology, Khartoum, Sudan., Dafaallah MM; Department of Microbiology, College of Medical Laboratory Sciences, Sudan University of Science and Technology, Khartoum, Sudan., Mohammed AA; Department of Pharmaceutical Biotechnology, College of Pharmacy, Ahfad University for Women, Omdurman, Khartoum, Sudan., El Hussein ARM; Department of Virology, Central Laboratory, Khartoum, Sudan., Hashim AI; Department of Microbiology, College of Medical Laboratory Sciences, Sudan University of Science and Technology, Khartoum, Sudan., Hamedelnil YF; Department of Microbiology, College of Medical Laboratory Sciences, Sudan University of Science and Technology, Khartoum, Sudan., Altayb HN; Biochemistry Department, Faculty of Sciences, King Abdulaziz University, Jeddah, Saudi Arabia. |
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Jazyk: | angličtina |
Zdroj: | F1000Research [F1000Res] 2020 Jul 27; Vol. 9, pp. 774. Date of Electronic Publication: 2020 Jul 27 (Print Publication: 2020). |
DOI: | 10.12688/f1000research.24818.2 |
Abstrakt: | Background: Pseudomonas aeruginosa is a pathogenic bacterium, causing nosocomial infections with intrinsic and acquired resistance mechanisms to a large group of antibiotics, including β-lactams. This study aimed to determine the susceptibility pattern to selected antibiotics and to index the first reported β-lactamases gene (extended spectrum β-lactamases (ESBLs) genes and class C β-lactamases genes) frequency in Ps. aeruginosa in Khartoum State, Sudan. Methods: 121 Ps. aeruginosa clinical isolates from various clinical specimens were used in this cross-sectional study conducted in Khartoum State. A total of 80 isolates were confirmed as Ps. aeruginosa through conventional identification methods and species-specific primers (the remaining 40 isolates were other bacterial species). The susceptibility pattern of the confirmed isolates to selected antibiotics was done following the Kirby Bauer disk diffusion method. Multiplex PCR was used for detection of seven β-lactamase genes ( bla Competing Interests: No competing interests were disclosed. (Copyright: © 2020 Abdelrahman DN et al.) |
Databáze: | MEDLINE |
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