Using dynein heavy chain 5 and creatine kinase levels in cervical fluid and blood for early diagnosing of ectopic pregnancy.

Autor: Sahin C; Department of Obstetrics and Gynecology, Ege University, Faculty of Medicine, Izmir, Turkey., Uygun ZO; Department of Biochemistry, Ege University, Faculty of Medicine, Izmir, Turkey., Hortu I; Department of Obstetrics and Gynecology, Ege University, Faculty of Medicine, Izmir, Turkey., Akdemir A; Department of Obstetrics and Gynecology, Ege University, Faculty of Medicine, Izmir, Turkey., Kocamanoglu M; Department of Biochemistry, Ege University, Faculty of Medicine, Izmir, Turkey., Ergenoglu AM; Department of Obstetrics and Gynecology, Ege University, Faculty of Medicine, Izmir, Turkey., Akcay Y; Department of Biochemistry, Ege University, Faculty of Medicine, Izmir, Turkey.
Jazyk: angličtina
Zdroj: The journal of obstetrics and gynaecology research [J Obstet Gynaecol Res] 2021 Mar; Vol. 47 (3), pp. 921-927. Date of Electronic Publication: 2020 Dec 17.
DOI: 10.1111/jog.14616
Abstrakt: Aim: Ectopic pregnancy is a life-threatening problem in reproductive ages. Diagnosing ectopic pregnancy in the early period provides to reducing mortality and morbidity and gives an opportunity for medical treatment to preserve fallopian tubes. Evaluation of cervical fluid for determining ectopic pregnancy with new promising markers provided different aspects for diagnosing ectopic pregnancy in the present study.
Methods: In this prospective clinical study, ectopic pregnant patients as ectopic pregnancy group (n = 46), intrauterine pregnant patients as intrauterine pregnancy group (n = 29) and not-pregnant patients as nonpregnancy group (n = 10) participated to study. Cervical fluid samples were collected with using merocel sponge. In addition, serum samples were obtained from patients. Dynein heavy chain 5 (DNAH5) and creatine kinase (CK) levels were determined by enzyme-linked immunosorbent assay kits in samples.
Results: Reduced cervical fluid DNAH5 levels was diagnosed in ectopic pregnancy group compared to intrauterine pregnancy group (median 3.42 ng/mL; 25-75% percentile 0-9.56 ng/mL vs median 6.14 ng/mL; 1.40-8.31 ng/mL; P < 0.001). On the other hand, DNAH5 protein was not detected in nonpregnant patients' samples. In addition, statistical significant increased cervical fluid CK levels were diagnosed in ectopic pregnancy group compared to intrauterine pregnancy group (median 4477.61 IU/L; 0-64 925.37 IU/L vs 0 IU/L; 0-6832.30 IU/L; P = 0.006).
Conclusion: Measuring of CK and DNAH5 in cervical fluid could be promising markers for early diagnosing of ectopic pregnancy. Decreased DNAH5 levels in cervical fluid might be result from abnormal cilia function in ectopic pregnant patients. ClinicalTrials.gov ID. NCT02995356.
(© 2020 Japan Society of Obstetrics and Gynecology.)
Databáze: MEDLINE
Externí odkaz: