Gene Networks Underlying the Resistance of Bifidobacterium longum to Inflammatory Factors.

Autor:	Veselovsky VA; Department of Molecular Biology and Genetics, Federal Research and Clinical Center of Physical-Chemical Medicine of Federal Medical Biological Agency, Moscow, Russia., Dyachkova MS; Department of Biotechnology, Vavilov Institute of General Genetics Russian Academy of Sciences, Moscow, Russia., Menyaylo EA; Phystech School of Biological and Medical Physics, Moscow Institute of Physics and Technology (National Research University), Dolgoprudny, Russia., Polyaeva PS; Phystech School of Biological and Medical Physics, Moscow Institute of Physics and Technology (National Research University), Dolgoprudny, Russia., Olekhnovich EI; Department of Molecular Biology and Genetics, Federal Research and Clinical Center of Physical-Chemical Medicine of Federal Medical Biological Agency, Moscow, Russia., Shitikov EA; Department of Molecular Biology and Genetics, Federal Research and Clinical Center of Physical-Chemical Medicine of Federal Medical Biological Agency, Moscow, Russia., Bespiatykh DA; Department of Molecular Biology and Genetics, Federal Research and Clinical Center of Physical-Chemical Medicine of Federal Medical Biological Agency, Moscow, Russia., Semashko TA; Department of Molecular Biology and Genetics, Federal Research and Clinical Center of Physical-Chemical Medicine of Federal Medical Biological Agency, Moscow, Russia., Kasianov AS; Department of Biotechnology, Vavilov Institute of General Genetics Russian Academy of Sciences, Moscow, Russia.; Phystech School of Biological and Medical Physics, Moscow Institute of Physics and Technology (National Research University), Dolgoprudny, Russia.; Laboratory of Plant Genomics, The Institute for Information Transmission Problems of the Russian Academy of Sciences (Kharkevich Institute), Moscow, Russia., Ilina EN; Department of Molecular Biology and Genetics, Federal Research and Clinical Center of Physical-Chemical Medicine of Federal Medical Biological Agency, Moscow, Russia., Danilenko VN; Department of Biotechnology, Vavilov Institute of General Genetics Russian Academy of Sciences, Moscow, Russia.; Faculty of Ecology, International Institute for Strategic Development of Sectoral Economics Peoples' Friendship University of Russia (RUDN University), Moscow, Russia., Klimina KM; Department of Molecular Biology and Genetics, Federal Research and Clinical Center of Physical-Chemical Medicine of Federal Medical Biological Agency, Moscow, Russia.; Department of Biotechnology, Vavilov Institute of General Genetics Russian Academy of Sciences, Moscow, Russia.
Jazyk:	angličtina
Zdroj:	Frontiers in immunology [Front Immunol] 2020 Nov 16; Vol. 11, pp. 595877. Date of Electronic Publication: 2020 Nov 16 (Print Publication: 2020).
DOI:	10.3389/fimmu.2020.595877
Abstrakt:	As permanent residents of the normal gut microbiota, bifidobacteria have evolved to adapt to the host's immune response whose priority is to eliminate pathogenic agents. The mechanisms that ensure the survival of commensals during inflammation and maintain the stability of the core component of the normal gut microbiota in such conditions remain poorly understood. We propose a new in vitro approach to study the mechanisms of resistance to immune response factors based on high-throughput sequencing followed by transcriptome analysis. This approach allowed us to detect differentially expressed genes associated with inflammation. In this study, we demonstrated that the presence of the pro-inflammatory cytokines IL-6 and TNFα to the growth medium of the B. longum subsp. longum GT15 strain changes the latter's growth rate insignificantly while affecting the expression of certain genes. We identified these genes and performed a COG and a KEGG pathway enrichment analysis. Using phylogenetic profiling we predicted the operons of genes whose expression was triggered by the cytokines TNFα and IL-6 in vitro . By mapping the transcription start points, we experimentally validated the predicted operons. Thus, in this study, we predicted the genes involved in a putative signaling pathway underlying the mechanisms of resistance to inflammatory factors in bifidobacteria. Since bifidobacteria are a major component of the human intestinal microbiota exhibiting pronounced anti-inflammatory properties, this study is of great practical and scientific relevance. (Copyright © 2020 Veselovsky, Dyachkova, Menyaylo, Polyaeva, Olekhnovich, Shitikov, Bespiatykh, Semashko, Kasianov, Ilina, Danilenko and Klimina.)
Databáze:	MEDLINE
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