Nanostructured Carbonated Hydroxyapatite Associated to rhBMP-2 Improves Bone Repair in Rat Calvaria.

Autor: Schneider Werner Vianna T; Pos-Graduation Program, Dentistry School, Universidade Federal Fluminense, Rio de Janeiro 24020-140, Brazil., Sartoretto SC; Oral Surgery Department, Universidade Veiga de Almeida, Rio de Janeiro 20271-020, Brazil.; Oral Surgery Department, Universidade Iguaçu, Rio de Janeiro 26260-045, Brazil., Neves Novellino Alves AT; Oral Diagnosis Department, Dentistry School, Universidade Federal Fluminense, Rio de Janeiro 24020-140, Brazil., Figueiredo de Brito Resende R; Oral Surgery Department, Universidade Iguaçu, Rio de Janeiro 26260-045, Brazil.; Oral Surgery Department, Dentistry School, Universidade Federal Fluminense, Rio de Janeiro 24020-140, Brazil., de Almeida Barros Mourão CF; Pos-Graduation Program, Dentistry School, Universidade Federal Fluminense, Rio de Janeiro 24020-140, Brazil., de Albuquerque Calasans-Maia J; Orthodontic Department, Dentistry School, Universidade Federal Fluminense, Rio de Janeiro 24020-140, Brazil., Martinez-Zelaya VR; Brazilian Synchrotron Light Laboratory (LNLS), Brazilian Center for Research in Energy and Materials (CNPEM), São Paulo 13083-970, Brazil., Malta Rossi A; Department of Condensed Matter, Applied Physics and Nanoscience, Brazilian Center for Research in Physics, Rio de Janeiro 22290-180, Brazil., Granjeiro JM; Pos-Graduation Program, Dentistry School, Universidade Federal Fluminense, Rio de Janeiro 24020-140, Brazil.; Directory of Life Sciences Applied Metrology, Instituto Nacional de Metrologia, Qualidade e Tecnologia (INMETRO), Duque de Caxias 25250-020, Brazil., Calasans-Maia MD; Oral Surgery Department, Dentistry School, Universidade Federal Fluminense, Rio de Janeiro 24020-140, Brazil., Seabra Louro R; Oral Surgery Department, Dentistry School, Universidade Federal Fluminense, Rio de Janeiro 24020-140, Brazil.
Jazyk: angličtina
Zdroj: Journal of functional biomaterials [J Funct Biomater] 2020 Dec 04; Vol. 11 (4). Date of Electronic Publication: 2020 Dec 04.
DOI: 10.3390/jfb11040087
Abstrakt: Many biomaterials are used for Bone Morphogenetic Proteins (BMPs) delivery in bone tissue engineering. The BMP carrier system's primary function is to hold these growth factors at the wound's site for a prolonged time and provide initial support for cells to attach and elaborate the extracellular matrix for bone regeneration. This study aimed to evaluate the nanostructured carbonated hydroxyapatite microspheres (nCHA) as an rhBMP-2 carrier on rats calvaria. A total of fifteen male Wistar rats were randomly divided into three groups ( n = 5): clot (control group), rhBMP-2 associated with collagen membrane (COL/rhBMP-2) or associated with the microspheres (nCHA/rhBMP-2). After 45 days, the calvaria defect samples were evaluated through histological, histomorphometric, and SR-µCT analyses to investigate new-formed bone and connective tissue volume densities. The descriptive histological analysis showed that nCHA/rhBMP-2 improved bone formation compared to other groups. These results were confirmed by histomorphometric and SR-µCT analysis that showed substantially defect area filling with a higher percentage of newly formed (36.24 ± 6.68) bone than those with the COL/rhBMP-2 (0.42 ± 0.40) and Clot (3.84 ± 4.57) ( p < 0.05). The results showed that nCHA is an effective carrier for rhBMP-2 encouraging bone healing and an efficient alternative to collagen membrane for rhBMP-2 delivery.
Databáze: MEDLINE