Liposomes Composed by Membrane Lipid Extracts from Macrophage Cell Line as a Delivery of the Trypanocidal N , N '-Squaramide 17 towards Trypanosoma cruzi .

Autor: Quijia CR; Microscopy Laboratory, Department of Cell Biology, Institute of Biology, University of Brasília, UnB-Brasilia, Federal District, Brasília DF 70910-900, Brazil.; Laboratory of Nanobiotechnology, Embrapa Genetic Resources and Biotechnology, Parque Estação Biológica, PqEB, Av. W5 Norte (Final) Caixa Postal 02372, Brasília DF 70.770-917, Brazil.; Pathogen-Host Interface Laboratory, Department of Cell Biology, Institute of Biology, University of Brasília, UnB-Brasilia, Federal District, Brasília DF 70910-900, Brazil., Bonatto CC; Laboratory of Nanobiotechnology, Embrapa Genetic Resources and Biotechnology, Parque Estação Biológica, PqEB, Av. W5 Norte (Final) Caixa Postal 02372, Brasília DF 70.770-917, Brazil., Silva LP; Laboratory of Nanobiotechnology, Embrapa Genetic Resources and Biotechnology, Parque Estação Biológica, PqEB, Av. W5 Norte (Final) Caixa Postal 02372, Brasília DF 70.770-917, Brazil., Andrade MA; Pathogen-Host Interface Laboratory, Department of Cell Biology, Institute of Biology, University of Brasília, UnB-Brasilia, Federal District, Brasília DF 70910-900, Brazil., Azevedo CS; Pathogen-Host Interface Laboratory, Department of Cell Biology, Institute of Biology, University of Brasília, UnB-Brasilia, Federal District, Brasília DF 70910-900, Brazil., Lasse Silva C; Pathogen-Host Interface Laboratory, Department of Cell Biology, Institute of Biology, University of Brasília, UnB-Brasilia, Federal District, Brasília DF 70910-900, Brazil., Vega M; Department of Chemistry, University of the Balearic Islands, Palma on the Island of Majorca, Carretera de Valldemossa, km 7.5, 07122 Palma, Illes Balears, Spain., de Santana JM; Pathogen-Host Interface Laboratory, Department of Cell Biology, Institute of Biology, University of Brasília, UnB-Brasilia, Federal District, Brasília DF 70910-900, Brazil., Bastos IMD; Pathogen-Host Interface Laboratory, Department of Cell Biology, Institute of Biology, University of Brasília, UnB-Brasilia, Federal District, Brasília DF 70910-900, Brazil., Carneiro MLB; Microscopy Laboratory, Department of Cell Biology, Institute of Biology, University of Brasília, UnB-Brasilia, Federal District, Brasília DF 70910-900, Brazil.
Jazyk: angličtina
Zdroj: Materials (Basel, Switzerland) [Materials (Basel)] 2020 Dec 02; Vol. 13 (23). Date of Electronic Publication: 2020 Dec 02.
DOI: 10.3390/ma13235505
Abstrakt: Chagas is a neglected tropical disease caused by Trypanosoma cruzi , and affects about 25 million people worldwide. N , N '-Squaramide 17 (S) is a trypanocidal compound with relevant in vivo effectiveness. Here, we produced, characterized, and evaluated cytotoxic and trypanocidal effects of macrophage-mimetic liposomes from lipids extracted of RAW 264.7 cells to release S. As results, the average hydrodynamic diameter and Zeta potential of mimetic lipid membranes containing S (MLS) was 196.5 ± 11 nm and -61.43 ± 2.3 mV, respectively. Drug entrapment efficiency was 73.35% ± 2.05%. After a 72 h treatment, MLS was observed to be active against epimastigotes in vitro (IC 50 = 15.85 ± 4.82 μM) and intracellular amastigotes (IC 50 = 24.92 ± 4.80 μM). Also, it induced low cytotoxicity with CC 50 of 1199.50 ± 1.22 μM towards VERO cells and of 1973.97 ± 5.98 μM in RAW 264.7. MLS also induced fissures in parasite membrane with a diameter of approximately 200 nm in epimastigotes. MLS showed low cytotoxicity in mammalian cells and high trypanocidal activity revealing this nanostructure a promising candidate for the development of Chagas disease treatment.
Databáze: MEDLINE
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