Unlocking Survival Mechanisms for Metal and Oxidative Stress in the Extremely Acidophilic, Halotolerant Acidihalobacter Genus.

Autor:	Khaleque HN; School of Pharmacy and Biomedical Sciences, Curtin University, Perth 6845, Australia.; CSIRO Land and Water, Floreat 6014, Australia., Fathollazadeh H; School of Pharmacy and Biomedical Sciences, Curtin University, Perth 6845, Australia., González C; Center for Bioinformatics and Genome Biology, Fundacion Ciencia y Vida, Santiago 7750000, Chile.; Centro de Genómica y Bioinformática, Facultad de Ciencias, Universidad Mayor, Santiago 8580000, Chile., Shafique R; School of Pharmacy and Biomedical Sciences, Curtin University, Perth 6845, Australia., Kaksonen AH; CSIRO Land and Water, Floreat 6014, Australia., Holmes DS; Center for Bioinformatics and Genome Biology, Fundacion Ciencia y Vida, Santiago 7750000, Chile.; Centro de Genómica y Bioinformática, Facultad de Ciencias, Universidad Mayor, Santiago 8580000, Chile.; Universidad San Sebastian, Santiago 8320000, Chile., Watkin ELJ; School of Pharmacy and Biomedical Sciences, Curtin University, Perth 6845, Australia.
Jazyk:	angličtina
Zdroj:	Genes [Genes (Basel)] 2020 Nov 24; Vol. 11 (12). Date of Electronic Publication: 2020 Nov 24.
DOI:	10.3390/genes11121392
Abstrakt:	Microorganisms used for the biohydrometallurgical extraction of metals from minerals must be able to survive high levels of metal and oxidative stress found in bioleaching environments. The Acidihalobacter genus consists of four species of halotolerant, iron-sulfur-oxidizing acidophiles that are unique in their ability to tolerate chloride and acid stress while simultaneously bioleaching minerals. This paper uses bioinformatic tools to predict the genes and mechanisms used by Acidihalobacter members in their defense against a wide range of metals and oxidative stress. Analysis revealed the presence of multiple conserved mechanisms of metal tolerance. Ac. yilgarnensis F5 T , the only member of this genus that oxidizes the mineral chalcopyrite, contained a 39.9 Kb gene cluster consisting of 40 genes encoding mobile elements and an array of proteins with direct functions in copper resistance. The analysis also revealed multiple strategies that the Acidihalobacter members can use to tolerate high levels of oxidative stress. Three of the Acidihalobacter genomes were found to contain genes encoding catalases, which are not common to acidophilic microorganisms. Of particular interest was a rubrerythrin genomic cluster containing genes that have a polyphyletic origin of stress-related functions.
Databáze:	MEDLINE
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