Varroa destructor mites vector and transmit pathogenic honey bee viruses acquired from an artificial diet.

Autor: Posada-Florez F; Bee Research Laboratory, Beltsville Agricultural Research Center, United States Department of Agriculture-Agricultural Research Service, Beltsville, Maryland, United States of America., Ryabov EV; Bee Research Laboratory, Beltsville Agricultural Research Center, United States Department of Agriculture-Agricultural Research Service, Beltsville, Maryland, United States of America., Heerman MC; Bee Research Laboratory, Beltsville Agricultural Research Center, United States Department of Agriculture-Agricultural Research Service, Beltsville, Maryland, United States of America., Chen Y; Bee Research Laboratory, Beltsville Agricultural Research Center, United States Department of Agriculture-Agricultural Research Service, Beltsville, Maryland, United States of America., Evans JD; Bee Research Laboratory, Beltsville Agricultural Research Center, United States Department of Agriculture-Agricultural Research Service, Beltsville, Maryland, United States of America., Sonenshine DE; Bee Research Laboratory, Beltsville Agricultural Research Center, United States Department of Agriculture-Agricultural Research Service, Beltsville, Maryland, United States of America.; Department of Biological Sciences, Old Dominion University, Norfolk, Virginia, United States of America., Cook SC; Bee Research Laboratory, Beltsville Agricultural Research Center, United States Department of Agriculture-Agricultural Research Service, Beltsville, Maryland, United States of America.
Jazyk: angličtina
Zdroj: PloS one [PLoS One] 2020 Nov 24; Vol. 15 (11), pp. e0242688. Date of Electronic Publication: 2020 Nov 24 (Print Publication: 2020).
DOI: 10.1371/journal.pone.0242688
Abstrakt: The ectoparasitic mite Varroa destructor is one of the most destructive pests of the honey bee (Apis mellifera) and the primary biotic cause of colony collapse in many regions of the world. These mites inflict physical injury on their honey bee hosts from feeding on host hemolymph and fat body cells/cellular components, and serve as the vector for deadly honey bee viruses, including Deformed wing virus (DWV) and the related Varroa destructor virus-1 (VDV-1) (i.e., DWV-like viruses). Studies focused on elucidating the dynamics of Varroa-mediated vectoring and transmission of DWV-like viruses may be confounded by viruses present in ingested host tissues or the mites themselves. Here we describe a system that includes an artificial diet free of insect tissue-derived components for maintaining Varroa mites for in vitro experimentation. Using this system, together with the novel engineered cDNA clone-derived genetically tagged VDV-1 and wild-type DWV, we demonstrated for the first time that Varroa mites provided an artificial diet supplemented with engineered viruses for 36 hours could acquire and transmit sufficient numbers of virus particles to establish an infection in virus-naïve hosts. While the in vitro system described herein provides for only up to five days of mite survival, precluding study of the long-term impacts of viruses on mite health, the system allows for extensive insights into the dynamics of Varroa-mediated vectoring and transmission of honey bee viruses.
Competing Interests: The authors have declared that no competing interests exist.
Databáze: MEDLINE
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