Protective potential of curcumin in L-NAME-induced hypertensive rat model: AT1R, mitochondrial DNA synergy.

Autor:	Greish SM; Department of Physiology, Faculty of Medicine, Suez Canal University Egypt.; Department of Physiology, School of Medicine, Badr University in Cairo Egypt., Abdel-Hady Z; Department of Histology and Cell Biology, Faculty of Medicine, Suez Canal University Egypt., Mohammed SS; Department of Histology and Cell Biology, Faculty of Medicine, Suez Canal University Egypt., Abdel-Hamed AR; Department of Biochemistry, Faculty of Pharmacy, Suez Canal University Egypt., Masoud RE; Department of Clinical Pharmacology, Faculty of Medicine, PortSaid University Egypt., Eltamany DA; Nutrition and Food Science, Home Economic Department, Faculty of Education, Suez Canal University Egypt., Abogresha NM; Department of Physiology, Faculty of Medicine, Suez Canal University Egypt.
Jazyk:	angličtina
Zdroj:	International journal of physiology, pathophysiology and pharmacology [Int J Physiol Pathophysiol Pharmacol] 2020 Oct 15; Vol. 12 (5), pp. 134-146. Date of Electronic Publication: 2020 Oct 15 (Print Publication: 2020).
Abstrakt:	Background & Objectives: Hypertension can be induced by inhibiting nitric oxide synthesis with L-NAME, which also has a role in oxidative stress. Curcumin has strong antioxidant property. Our aim was to examine the possible preventive role of curcumin on renal dysfunction secondary to hypertension. Material & Methods: Twenty-four adult male Albino rats were divided in four groups: normal (N); curcumin (C; received curcumin 100 mg/kg/day by oral gavage for 10 weeks); hypertensive (H; received L-NAME 40 mg/kg/day in their drinking water for 4 weeks); and hypertensive-curcumin (HC; received L-NAME and curcumin). Arterial blood pressure was evaluated non-invasively for 4 weeks. Rats were then sacrificed for assessment of oxidative stress (catalase, lipid peroxidase, reduced glutathione and superoxide dismutase), renal function and structure, and biomarkers of apoptosis (Bcl-2 and caspase-3). AT1R expression and renal mtDNA integrity were also assessed. Results: Curcumin attenuated the effects of L-NAME on blood pressure and renal function. The renal histopathological changes observed in the L-NAME group were improved by curcumin administration. The expression of Bcl2 and caspase-3 was improved associated with downregulation of AT1R in curcumin treated groups. The antioxidant markers and mtDNA fragmentation show marked increase in hypertensive group which significantly decreased after curcumin treatment. Conclusion: Curcumin improved blood pressure elevation renal dysfunction. These improvements mediated through anti-oxidant capabilities and downregulation of AT1R favoring reduced apoptosis and preserved mitochondrial DNA. Competing Interests: None. (IJPPP Copyright © 2020.)
Databáze:	MEDLINE
Externí odkaz:	Zobrazit plný text záznamu