| Autor: |
Nobles CL; Department of Microbiology, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA. chris.l.nobles@gmail.com. |
| Jazyk: |
angličtina |
| Zdroj: |
Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2021; Vol. 2189, pp. 71-80. |
| DOI: |
10.1007/978-1-0716-0822-7_6 |
| Abstrakt: |
With the advent of genome editing technologies, scientists have recognized that these technologies can be prone to nonspecific or off-target activity. As many areas of the genome are sensitive and can give rise to abnormalities if mutated, it is imperative that scientists identify regions of off-target activity in order to utilize these new technologies for medical benefits. GUIDE-seq and iGUIDE both use an oligo-based marker method to identify regions of DNA double-strand breaks in an unbiased manner. The repeated observation of these double-strand breaks across the genome in comparison with target sequences (such as guide RNAs) has allowed researchers to identify on- and off-target sites related to their targeted-nuclease technologies. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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