| Autor: |
Ambrose B; Sheffield Institute for Nucleic Acids, Department of Chemistry, University of Sheffield, Sheffield, UK., Baxter JM; Sheffield Institute for Nucleic Acids, Department of Chemistry, University of Sheffield, Sheffield, UK., Cully J; Sheffield Institute for Nucleic Acids, Department of Chemistry, University of Sheffield, Sheffield, UK., Willmott M; Sheffield Institute for Nucleic Acids, Department of Chemistry, University of Sheffield, Sheffield, UK., Steele EM; Department of Physics, University of Sheffield, Sheffield, UK., Bateman BC; Central Laser Facility, Research Complex at Harwell, Rutherford Appleton Laboratory, Oxford, UK., Martin-Fernandez ML; Central Laser Facility, Research Complex at Harwell, Rutherford Appleton Laboratory, Oxford, UK., Cadby A; Department of Physics, University of Sheffield, Sheffield, UK., Shewring J; Sheffield Institute for Nucleic Acids, Department of Chemistry, University of Sheffield, Sheffield, UK., Aaldering M; Sheffield Institute for Nucleic Acids, Department of Chemistry, University of Sheffield, Sheffield, UK., Craggs TD; Sheffield Institute for Nucleic Acids, Department of Chemistry, University of Sheffield, Sheffield, UK. t.craggs@sheffield.ac.uk. |
| Abstrakt: |
Single-molecule Förster Resonance Energy Transfer (smFRET) is a powerful technique capable of resolving both relative and absolute distances within and between structurally dynamic biomolecules. High instrument costs, and a lack of open-source hardware and acquisition software have limited smFRET's broad application by non-specialists. Here, we present the smfBox, a cost-effective confocal smFRET platform, providing detailed build instructions, open-source acquisition software, and full validation, thereby democratising smFRET for the wider scientific community. |
