| Autor: |
Silva JRD; Instituto Federal de Educação, Ciência e Tecnologia do Piauí - IFPI, Teresina, PI, Brazil., Pais AKL; Universidade Federal Rural de Pernambuco - UFRPE, Departamento de Agronomia, Recife, PE, Brazil., Albuquerque GMR; Universidade Federal Rural de Pernambuco - UFRPE, Departamento de Agronomia, Recife, PE, Brazil., Silva AMF; Universidade Federal de Alagoas - UFAL, Departamento de Agronomia, Maceió, AL, Brazil., Silva Junior WJ; Universidade Federal de Pernambuco - UFPE, Departamento de Genética, Recife, PE, Brazil., Balbino VQ; Universidade Federal de Pernambuco - UFPE, Departamento de Genética, Recife, PE, Brazil., Fonseca MEN; Embrapa Hortaliças, Brasília, DF, Brazil., Gama MASD; Universidade Federal Rural de Pernambuco - UFRPE, Departamento de Agronomia, Recife, PE, Brazil., Souza EB; Universidade Federal Rural de Pernambuco - UFRPE, Departamento de Biologia, Recife, PE, Brazil., Mariano RLR; Universidade Federal Rural de Pernambuco - UFRPE, Departamento de Agronomia, Recife, PE, Brazil. |
| Abstrakt: |
Ralstonia solanacearum is the causal agent of Moko disease in bananas, which in the state of Sergipe in northeastern Brazil causes "Sergipe facies". This disease induces atypical symptoms similar to those of Bugtok disease in the Philippines. This study was conducted to sequence, assemble, and annotate the genomes of the Sergipe facies-causing isolates SFC and IBSBF2570 (sequevar IIA-53) and compare their genomes with two representative isolates causing Bugtok disease. The genomes were sequenced and assembled, resulting in lengths of 5.58 Mb (SFC) and 5.46 Mb (IBSBF2570) in 185 and 174 contigs, respectively. The isolates of Sergipe facies and Bugtok disease showed similarities in their gene contents. We identified 5,668 information clusters, 3,752 of which were shared by all genomes (core genes). Moreover, 3,585 single-copy genes were identified. Isolates causing Bugtok disease exclusively shared 266 more information clusters than the isolates causing Sergipe facies. These results suggest that Sergipe facies and Bugtok disease isolates show high genomic similarity. However, the similarity is even greater between the Bugtok disease isolates. This may be because of their longer period of interaction compared to Sergipe facies isolates. |
