Acetylcholinesterase inhibitory activity of Ocotea pomaderroides extracts: HPLC-MS/MS characterization and molecular modeling studies.
| Autor: | Reis IMA; Laboratory of Phytochemistry, Health Department, State University of Feira de Santana, Feira de Santana, Bahia, Brazil., Cassiano DSA; Laboratory of Phytochemistry, Health Department, State University of Feira de Santana, Feira de Santana, Bahia, Brazil., Conceição RS; Laboratory of Phytochemistry, Health Department, State University of Feira de Santana, Feira de Santana, Bahia, Brazil., Freitas HF; Bioinformatics and Molecular Modeling Laboratory (LaBiMM), Pharmacy College, Federal University of Bahia, Salvador, Bahia, Brazil.; Post Graduation program on Pharmacy, Pharmacy College, Federal University of Bahia, Salvador, Bahia, Brazil., Pita SSDR; Bioinformatics and Molecular Modeling Laboratory (LaBiMM), Pharmacy College, Federal University of Bahia, Salvador, Bahia, Brazil., David JM; Post Graduation program on Pharmacy, Pharmacy College, Federal University of Bahia, Salvador, Bahia, Brazil.; Chemistry Institute, Federal University of Bahia, Salvador, Bahia, Brazil., Branco A; Laboratory of Phytochemistry, Health Department, State University of Feira de Santana, Feira de Santana, Bahia, Brazil. |
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| Jazyk: | angličtina |
| Zdroj: | Natural product research [Nat Prod Res] 2022 Feb; Vol. 36 (4), pp. 999-1003. Date of Electronic Publication: 2020 Nov 04. |
| DOI: | 10.1080/14786419.2020.1839453 |
| Abstrakt: | The present work describes the acetylcholinesterase inhibitory activity of Ocotea pomaderroides extracts besides the chemical composition of chromatographic fractions. The hexane, dichloromethane and ethyl acetate extract soluble fractions showed high Electrophorus electricus acetylcholinesterase ( Eel AChE) inhibition (92.18, 71.86 and 74.25%, respectively) while the butanolic and aqueous extracts showed moderate to low activities (44.48 and 20.74%, respectively). The high-performance liquid chromatography coupled with electrospray ionization multiple-stage mass spectrometry (HPLC-ESI-MS n ) analysis led to the identification of the alkaloids and flavonol glycoside derivatives present in these extracts. The binding profile of the alkaloids and their atomic effect on 3D structure of Electrophorus electricus AchE (EelAChE) were assessed with molecular modeling. |
| Databáze: | MEDLINE |
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