| Autor: |
Oriero EC; 1Medical Research Council Unit The Gambia at LSHTM, Banjul, The Gambia., Olukosi AY; 2Nigerian Institute of Medical Research (NIMR), Lagos, Nigeria., Oduwole OA; 3Calabar Institute of Tropical Disease Research and Prevention, University of Calabar Teaching Hospital, Calabar, Nigeria., Djimde A; 4Department of Epidemiology of Parasitic Diseases, Malaria Research and Training Center, University of Science, Techniques and Technology of Bamako, Bamako, Mali., D'Alessandro U; 1Medical Research Council Unit The Gambia at LSHTM, Banjul, The Gambia., Meremikwu MM; 3Calabar Institute of Tropical Disease Research and Prevention, University of Calabar Teaching Hospital, Calabar, Nigeria., Amambua-Ngwa A; 1Medical Research Council Unit The Gambia at LSHTM, Banjul, The Gambia. |
| Jazyk: |
angličtina |
| Zdroj: |
The American journal of tropical medicine and hygiene [Am J Trop Med Hyg] 2020 Dec; Vol. 103 (6), pp. 2208-2216. Date of Electronic Publication: 2020 Oct 22. |
| DOI: |
10.4269/ajtmh.20-0593 |
| Abstrakt: |
Although Plasmodium falciparum continues to be the main target for malaria elimination, other Plasmodium species persist in Africa. Their clinical diagnosis is uncommon, whereas rapid diagnostic tests (RDTs), the most widely used malaria diagnostic tools, are only able to distinguish between P. falciparum and non- falciparum species, the latter as "pan-species." Blood samples from health facilities were collected in southern Nigeria (Lagos and Calabar) in 2017 (October-December) and Calabar only in 2018 (October-November), and analyzed by several methods, namely, microscopy, quantitative real-time PCR (qPCR), and peptide serology targeting candidate antigens ( Plasmodium malariae apical membrane antigen, P . malariae lactose dehydrogenase, and P . malariae circumsporozoite surface protein). Both microscopy and qPCR diagnostic approaches detected comparable proportions (∼80%) of all RDT-positive samples infected with the dominant P. falciparum malaria parasite. However, higher proportions of non- falciparum species were detected by qPCR than microscopy, 10% against 3% infections for P. malariae and 3% against 0% for Plasmodium ovale , respectively. No Plasmodium vivax infection was detected. Infection rates for P. malariae varied between age-groups, with the highest rates in individuals aged > 5 years. Plasmodium malariae -specific seroprevalence rates fluctuated in those aged < 10 years but generally reached the peak around 20 years of age for all peptides. The heterogeneity and rates of these non-falciparum species call for increased specific diagnosis and targeting by elimination strategies. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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