From Differential Stains to Next Generation Physiology: Chemical Probes to Visualize Bacterial Cell Structure and Physiology.

Autor: Hira J; Research Group for Host-Microbe Interactions, Department of Medical Biology and Centre for New Antibacterial Strategies (CANS), UiT-The Arctic University of Norway, 9019 Tromsø, Norway., Uddin MJ; Research Group for Host-Microbe Interactions, Department of Medical Biology and Centre for New Antibacterial Strategies (CANS), UiT-The Arctic University of Norway, 9019 Tromsø, Norway., Haugland MM; Department of Chemistry and Centre for New Antibacterial Strategies (CANS), UiT-The Arctic University of Norway, 9019 Tromsø, Norway., Lentz CS; Research Group for Host-Microbe Interactions, Department of Medical Biology and Centre for New Antibacterial Strategies (CANS), UiT-The Arctic University of Norway, 9019 Tromsø, Norway.
Jazyk: angličtina
Zdroj: Molecules (Basel, Switzerland) [Molecules] 2020 Oct 26; Vol. 25 (21). Date of Electronic Publication: 2020 Oct 26.
DOI: 10.3390/molecules25214949
Abstrakt: Chemical probes have been instrumental in microbiology since its birth as a discipline in the 19th century when chemical dyes were used to visualize structural features of bacterial cells for the first time. In this review article we will illustrate the evolving design of chemical probes in modern chemical biology and their diverse applications in bacterial imaging and phenotypic analysis. We will introduce and discuss a variety of different probe types including fluorogenic substrates and activity-based probes that visualize metabolic and specific enzyme activities, metabolic labeling strategies to visualize structural features of bacterial cells, antibiotic-based probes as well as fluorescent conjugates to probe biomolecular uptake pathways.
Databáze: MEDLINE
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