3D Printed Gene-activated Octacalcium Phosphate Implants for Large Bone Defects Engineering.

Autor:	Bozo IY; Department of Maxillofacial Surgery, A.I. Burnazyan Federal Medical Biophysical Center, FMBA of Russia, Moscow, Russia.; Research and Development Department, Human Stem Cells Institute, Moscow, Russia., Deev RV; Research and Development Department, Human Stem Cells Institute, Moscow, Russia.; Department of Pathology, I.I. Mechnikov North-Western State Medical University, Saint-Petersburg, Russia., Smirnov IV; A.A. Baikov Institute of Metallurgy and Materials Science, Russian Academy of Sciences, Moscow, Russia., Fedotov AY; A.A. Baikov Institute of Metallurgy and Materials Science, Russian Academy of Sciences, Moscow, Russia., Popov VK; Institute of Photon Technologies of Federal Scientific Research Centre 'Crystallography and Photonics,' Russian Academy of Sciences, Moscow, Russia., Mironov AV; Institute of Photon Technologies of Federal Scientific Research Centre 'Crystallography and Photonics,' Russian Academy of Sciences, Moscow, Russia., Mironova OA; Institute of Photon Technologies of Federal Scientific Research Centre 'Crystallography and Photonics,' Russian Academy of Sciences, Moscow, Russia., Gerasimenko AY; Institute for Bionic Technologies and Engineering, I.M. Sechenov First Moscow State Medical University, Moscow, Russia.; Institute of Biomedical Systems, National Research University of Electronic Technology, Moscow, Russia., Komlev VS; A.A. Baikov Institute of Metallurgy and Materials Science, Russian Academy of Sciences, Moscow, Russia.; Institute of Photon Technologies of Federal Scientific Research Centre 'Crystallography and Photonics,' Russian Academy of Sciences, Moscow, Russia.
Jazyk:	angličtina
Zdroj:	International journal of bioprinting [Int J Bioprint] 2020 Jun 03; Vol. 6 (3), pp. 275. Date of Electronic Publication: 2020 Jun 03 (Print Publication: 2020).
DOI:	10.18063/ijb.v6i3.275
Abstrakt:	The aim of the study was the development of three-dimensional (3D) printed gene-activated implants based on octacalcium phosphate (OCP) and plasmid DNA encoding VEGFA . The first objective of the present work involved design and fabrication of gene-activated bone substitutes based on the OCP and plasmid DNA with VEGFA gene using 3D printing approach of ceramic constructs, providing the control of its architectonics compliance to the initial digital models. X-ray diffraction, scanning electron microscopy (SEM), Fourier transform infrared spectroscopy, and compressive strength analyses were applied to investigate the chemical composition, microstructure, and mechanical properties of the experimental samples. The biodegradation rate and the efficacy of plasmid DNA delivery in vivo were assessed during standard tests with subcutaneous implantation to rodents in the next stage. The final part of the study involved substitution of segmental tibia and mandibular defects in adult pigs with 3D printed gene-activated implants. Biodegradation, osteointegration, and effectiveness of a reparative osteogenesis were evaluated with computerized tomography, SEM, and a histological examination. The combination of gene therapy and 3D printed implants manifested the significant clinical potential for effective bone regeneration in large/critical size defect cases. Competing Interests: The authors declare that they have no conflicts of interest. (Copyright: © 2020 Bozo, et al.)
Databáze:	MEDLINE
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