The regulation of trophoblast invasion and decidual reaction by matrix metalloproteinase-2, metalloproteinase-7, and metalloproteinase-9 expressions in the rat endometrium.

Autor: Hamutoğlu R; Department of Histology and Embryology Faculty of Medicine Cumhuriyet University Sivas Turkey., Bulut HE; Department of Histology and Embryology Faculty of Medicine Cumhuriyet University Sivas Turkey., Kaloğlu C; Department of Histology and Embryology Faculty of Medicine Cumhuriyet University Sivas Turkey.; Cumhuriyet University Assisted Reproduction Technology (ART) Center Sivas Turkey., Önder O; Department of Histology and Embryology Faculty of Medicine Cumhuriyet University Sivas Turkey., Dağdeviren T; Department of Histology and Embryology Faculty of Medicine Cumhuriyet University Sivas Turkey., Aydemir MN; Department of Molecular Biology and Genetics Faculty of Science Cumhuriyet University Sivas Turkey., Korkmaz EM; Department of Molecular Biology and Genetics Faculty of Science Cumhuriyet University Sivas Turkey.
Jazyk: angličtina
Zdroj: Reproductive medicine and biology [Reprod Med Biol] 2020 Aug 06; Vol. 19 (4), pp. 385-397. Date of Electronic Publication: 2020 Aug 06 (Print Publication: 2020).
DOI: 10.1002/rmb2.12342
Abstrakt: Purpose: We aimed to evaluate how matrix metalloproteinases (MMPs) regulate the trophoblast invasion and placentation.
Methods: Female rats were divided into the estrous cycle and early pregnancy day groups. Obtained uterine tissues and implantation sites were processed for immunofluorescence and real-time PCR examinations.
Results: The mRNA expression of MMP-7 was higher than MMP-2 and MMP-9. Immunofluorescence findings confirmed that MMP-2, MMP-7, and MMP-9 were localized in the endometrial stroma, while MMP-7 was high in glandular and lining epithelial cells throughout the entire estrous cycle. However, their immunolocalizations and mRNA expressions were dramatically changed with the early pregnancy days. The MMP-7 reached very strong immunostaining in the giant trophoblast cells (GTCs), and the cytoplasm of mature and differentiating decidual cells, whereas MMP-2 and MMP-9 were mostly seen in the primary decidual zone (PDZ), GTCs, and the endothelium of blood vessels.
Conclusions: All three MMPs seemed likely to be a key mediator of trophoblast invasion into the decidual region as well as angiogenesis during the placentation process. Due to the strong and wide expression of MMP-7 in the mature decidua, it could be suggested that MMP-7 is important for decidual ECM remodeling and it might be used as a new marker of decidual reaction.
Competing Interests: Conflicts of Interest: The authors declare that there is no conflict of interest that could be perceived as prejudicing the impartiality of the research reported. Human and animal rights: This article does not describe any experiments involving human participants. All of the institutional and national guidelines for the care and use of laboratory animals were followed. The protocol for the research project was approved by a suitably constituted ethics committee.
(© 2020 The Authors. Reproductive Medicine and Biology published by John Wiley & Sons Australia, Ltd on behalf of Japan Society for Reproductive Medicine.)
Databáze: MEDLINE
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