Prevention of LPS-induced acute respiratory distress syndrome in sheep by bone marrow-derived mesenchymal stem/stromal cells.

Autor: Sadeghian Chaleshtori S; Department of Internal Medicine, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran; Institute of Biomedical Research, University of Tehran, Tehran, Iran. Electronic address: s.sadeghian@ut.ac.ir., Mokhber Dezfouli MR; Department of Internal Medicine, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran; Institute of Biomedical Research, University of Tehran, Tehran, Iran. Electronic address: mokhberd@ut.ac.ir., Abbasi J; Graduate of Residency in Large Animal Internal Medicine, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran., Dehghan MM; Department of Surgery and Radiology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran; Institute of Biomedical Research, University of Tehran, Tehran, Iran., Jabbari Fakhr M; Department of Tissue Engineering, Faculty of Medicine, Qom University of Medical Sciences, Qom, Iran; Institute of Biomedical Research, University of Tehran, Tehran, Iran., Yadollahi S; Graduated in Doctorate of Veterinary Medicine, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran., Mirabad MM; Graduate of Residency in Large Animal Internal Medicine, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran.
Jazyk: angličtina
Zdroj: Life sciences [Life Sci] 2020 Dec 15; Vol. 263, pp. 118600. Date of Electronic Publication: 2020 Oct 14.
DOI: 10.1016/j.lfs.2020.118600
Abstrakt: Materials and Methods: In this study, 10 male Shall sheep were used in two groups and bone marrow samples were collected and BM-MSCs isolated. Then experimental model of ARDS was induced by intrapulmonary injection of LPS to dose of 400 μg/kg. Twenty-four hours after LPS injection, 5 × 10 7 cells of BM-MSCs were autologous transferred in the group of treatment and 1 ml PBS was infused in the group of control as intrapulmonary. Then, the symptoms of clinical, complete blood count, analysis of arterial blood gases and the concentrations of IL6,IL10,TNF-α,total protein, Ig M and albumin BAL were determined before and at times of 3,6,12,24,48,72, and 168 after transplantation/infusion.
Key Findings: The results of the investigations 24 h post-LPS injection(time 0) indicated the occurrence of acute inflammation which confirmed ARDS model. These changes included increase in RR, HR and RT, decrease in PO2 and SatO2 and increase in PCO2, WBC, neutrophils, macrophages, total protein,IL6,IL10, TNF-α,Ig M and albumin. But the stem/stromal cells transplantation reduced the severity of clinical signs induced by LPS, caused significant increase in PO 2 , SatO 2 and IL-10 and significant decrease in PCO 2 , the total protein, TNF-α,IL-6, Ig M, albumin, WBCs, neutrophils and macrophages at different times of sampling both in compared with before transplantation(time 0) and in compared with the group of control. While in the control group, inflammation continued until the end of the study.
Significance: These results showed that BM-MSCs are able to reduce inflammation and have an important role in reconstruction of the damaged lung.
(Copyright © 2020 Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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