| Autor: |
Melo FD; Universidade do Estado de Santa Catarina/UDESC, Centro de Ciências Agroveterinárias, Avenida Luiz de Camões, 2090, Conta Dinheiro, 88520-000 Lages, SC, Brazil., Sfaciotte RAP; Universidade do Estado de Santa Catarina/UDESC, Centro de Ciências Agroveterinárias, Avenida Luiz de Camões, 2090, Conta Dinheiro, 88520-000 Lages, SC, Brazil., Dalmina KA; Universidade do Estado de Santa Catarina/UDESC, Centro de Ciências Agroveterinárias, Avenida Luiz de Camões, 2090, Conta Dinheiro, 88520-000 Lages, SC, Brazil., Wildemann P; Universidade do Estado de Santa Catarina/UDESC, Centro de Ciências Agroveterinárias, Avenida Luiz de Camões, 2090, Conta Dinheiro, 88520-000 Lages, SC, Brazil., Parussolo L; Instituto Federal de Santa Catarina/IFSC, Campus Florianópolis, Avenida Mauro Ramos, 950, Centro, 88020-300 Florianópolis, SC, Brazil., Wosiacki SR; Universidade Estadual de Maringa/UEM, Estrada da Paca s/nº, São Cristóvão, 87502-970 Umuarama, PR, Brazil., Costa UMD; Universidade do Estado de Santa Catarina/UDESC, Centro de Ciências Agroveterinárias, Avenida Luiz de Camões, 2090, Conta Dinheiro, 88520-000 Lages, SC, Brazil., Ferraz SM; Universidade do Estado de Santa Catarina/UDESC, Centro de Ciências Agroveterinárias, Avenida Luiz de Camões, 2090, Conta Dinheiro, 88520-000 Lages, SC, Brazil. |
| Abstrakt: |
In this work, we investigated the phenotypic profile of Staphylococcus spp. isolates recovered from raw milk and artisanal cheese, and their enterotoxigenic potential through the detection of classical enterotoxin genes (sea, seb, sec, sed and see). A total of 104 isolates (58 coagulase-positive Staphylococcus - CoPS; and 46 coagulase-negative Staphylococcus- CoNS) were used, of which 33 were retrieved from raw milk and 71 from artisanal cheese produced in the Serrana region of Santa Catarina. Identification of CoPS was conducted via biochemical tests. Detection of the genes sea, seb, sec, sed, and see was carried out by multiplex PCR technique. Among the 58 CoPS analyzed, 64% were identified as S. aureus, 22% as S. scheiferi coagulans, 12% as S. hyicus and as 2% S. intermedius. In the present study was noted that 40% of CoPS isolates retrieved from milk harbored seb gene, while only one from artisanal cheese was positive for gene sea. In this study all CoNS samples investigated were negative for enterotoxins genes. The enterotoxigenic potential of CoPS, is an issue of great importance for public health. For that reason, it is necessary that cheese factories strictly follow the safety processes involved in manufacturing. |
