| Autor: |
Sayed AM; Department of Pharmacognosy, Faculty of Pharmacy, Nahda University, Beni-Suef, Egypt., Sherif NH; Department of Pharmacognosy, Faculty of Pharmacy, Nahda University, Beni-Suef, Egypt.; Drug Radiation Research Department, National Center for Radiation Research and Technology, Atomic Energy Authority, Egypt., El-Gendy AO; Department of Microbiology, Faculty of Pharmacy, Beni-Suef University, Beni-Suef, Egypt., Shamikh YI; Department of Microbiology and Immunology, Nahda University, Beni-Suef, Egypt.; Department of Virology, Egypt Center for Research and Regenerative Medicine (ECRRM), Beni Suef, Egypt., Ali AT; Department of Biochemistry, Faculty of Pharmacy, Nahda University, Beni-Suef, Egypt., Attia EZ; Department of Pharmacognosy, Faculty of Pharmacy, Minia University, Minia, Egypt., El-Katatny MH; Department of Botany and Microbiology, Faculty of Science, Minia University, Minia, Egypt., Khalifa BA; Department of Botany and Microbiology, Faculty of Science, Minia University, Minia, Egypt., Hassan HM; Department of Pharmacognosy, Faculty of Pharmacy, Beni-Suef University, Beni-Suef, Egypt., Abdelmohsen UR; Department of Pharmacognosy, Faculty of Pharmacy, Minia University, Minia, Egypt.; Department of Pharmacognosy, Faculty of Pharmacy, Deraya University, New Minia, Egypt. |
| Abstrakt: |
In this work, three fungal endophytes identified as Aspergillus terreus (AFL, AFSt and AFR), were studied for their antioxidant potential. LC-MS-based metabolomics, followed by multivariate statistical analysis were then applied to comprehensively profile their extracts. The three fungal endophytes revealed interesting antioxidant potential, in particular, the strain isolated from the Artemisia annua leaves (AFL), which was rich in different types of phenolic metabolites. Additionally, all fungal-derived ethyl acetate extracts showed potent inhibition against the prooxidant xanthine oxidase. Multivariate analysis (PCA and PLS-DA) demonstrated a unique chemical fingerprint for each strain, where phenolics, coumarins, and polyketides were the discriminative metabolites of the three fungal strains. The present findings highlighted the power of metabolomics in the chemotaxonomical classification of closely related strains. It also asserted the role of fungal endophytes in the management of oxidative stress, particularly when they are utilized in the production of fermented food products. |
