Recombinant Spidroin Films Attenuate Individual Markers of Glucose Induced Aging in NIH 3T3 Fibroblasts.

Autor: Moysenovich AM; Faculty of Biology, Lomonosov Moscow State University, Moscow, 119234, Russia., Moisenovich MM; Faculty of Biology, Lomonosov Moscow State University, Moscow, 119234, Russia. mmoisenovich@mail.ru., Sudina AK; Faculty of Biology, Lomonosov Moscow State University, Moscow, 119234, Russia., Tatarskiy VV; Blokhin National Medical Research Center of Oncology, Moscow, 115478, Russia.; Institute of Gene Biology, Russian Academy of Sciences, Moscow, 119334, Russia.; National University of Science and Technology 'MISiS', Moscow, 119049, Russia., Khamidullina AI; Institute of Gene Biology, Russian Academy of Sciences, Moscow, 119334, Russia., Yastrebova MA; Institute of Gene Biology, Russian Academy of Sciences, Moscow, 119334, Russia., Davydova LI; NRC 'Kurchatov Institute' - GOSNIIGENETIKA, Moscow, 117519, Russia.; NRC 'Kurchatov Institute', Moscow, 123182, Russia., Bogush VG; NRC 'Kurchatov Institute' - GOSNIIGENETIKA, Moscow, 117519, Russia.; NRC 'Kurchatov Institute', Moscow, 123182, Russia., Debabov VG; NRC 'Kurchatov Institute' - GOSNIIGENETIKA, Moscow, 117519, Russia.; NRC 'Kurchatov Institute', Moscow, 123182, Russia., Arkhipova AY; Faculty of Biology, Lomonosov Moscow State University, Moscow, 119234, Russia.; Moscow Regional Research and Clinical Institute (MONIKI), Moscow, 129110, Russia., Shaitan KV; Faculty of Biology, Lomonosov Moscow State University, Moscow, 119234, Russia.; Semenov Institute of Chemical Physics, Russian Academy of Sciences, Moscow, 119991, Russia., Shtil AA; Blokhin National Medical Research Center of Oncology, Moscow, 115478, Russia.; Institute of Gene Biology, Russian Academy of Sciences, Moscow, 119334, Russia., Demina IA; Faculty of Biology, Lomonosov Moscow State University, Moscow, 119234, Russia.; Dmitry Rogachev National Medical Research Center of Pediatric Hematology, Oncology and Immunology, Moscow, 117198, Russia.
Jazyk: angličtina
Zdroj: Biochemistry. Biokhimiia [Biochemistry (Mosc)] 2020 Jul; Vol. 85 (7), pp. 808-819.
DOI: 10.1134/S0006297920070093
Abstrakt: The effect of bioresorbable materials on aging in cultured mouse NIH 3T3 fibroblasts treated with elevated glucose concentration was investigated. The cells were grown on films produced from the silkworm fibroin and rS1/9, a recombinant analog of Nephila clavipes spidroin 1. Exposure to 50 mM glucose of the cells grown on uncoated glass support resulted in the cell growth retardation. The average areas of the cells and nuclei and the percentage of apoptotic cells increased, whereas the amount of soluble collagen decreased. In contrast, on the fibroin and spidroin films, the cell density and the percentage of 5-bromo-2'-deoxyuridine (BrdU)-positive cells were higher vs. the cells grown on the glass support. The films protected NIH 3T3 fibroblasts from the glucose-induced death. The most prominent effects on the cell density, BrdU incorporation, and apoptosis prevention were observed in the cells cultured on spidroin films. Unlike the cells grown on glass support (decrease in the soluble collagen production) or fibroin (no effect), production of soluble collagen by the cells grown on spidroin films increased after cell exposure to 50 mM glucose. Molecular analysis demonstrated that 50 mM glucose upregulated phosphorylation of the NFκB heterodimer p65 subunit in the cells grown on the glass support. The treatment of cells grown on fibroin films with 5.5 mM or 50 mM glucose had no effect on p65 phosphorylation. The same treatment decreased p65 phosphorylation in the cells on the spidroin films. These results demonstrate the anti-aging efficacy of biomaterials derived from the silk proteins and suggest that spidroin is more advantageous for tissue engineering and therapy than fibroin.
Databáze: MEDLINE
Externí odkaz:
Nepřihlášeným uživatelům se plný text nezobrazuje