Comparison of Primer-Probe Sets among Different Master Mixes for Laboratory Screening of Severe Acute Respiratory Syndrome Coronavirus 2 ( SARS-CoV-2 ).

Autor: Cuong HQ; Directorial board, Pasteur Institute in Ho Chi Minh City, Vietnam., Hai ND; Planning Division, Pasteur Institute in Ho Chi Minh City, Vietnam., Linh HT; Medical Analysis Department, Pasteur Institute in Ho Chi Minh City, Vietnam., Anh NH; Microbiology and Immunology Department, Pasteur Institute in Ho Chi Minh City, Vietnam., Hieu NT; Microbiology and Immunology Department, Pasteur Institute in Ho Chi Minh City, Vietnam., Thang CM; Microbiology and Immunology Department, Pasteur Institute in Ho Chi Minh City, Vietnam., Thao NTT; Microbiology and Immunology Department, Pasteur Institute in Ho Chi Minh City, Vietnam., Lan PT; Directorial board, Pasteur Institute in Ho Chi Minh City, Vietnam.
Jazyk: angličtina
Zdroj: BioMed research international [Biomed Res Int] 2020 Sep 25; Vol. 2020, pp. 7610678. Date of Electronic Publication: 2020 Sep 25 (Print Publication: 2020).
DOI: 10.1155/2020/7610678
Abstrakt: Background: There is a shortage of chemical reagents for severe acute respiratory syndrome coronavirus 2 ( SARS-CoV-2 ) diagnosis and a surge of SARS-CoV-2 cases, especially in limited-resource settings. Therefore, the combination of an optimal assay kit is necessary.
Methods: We compared the ability to screen SARS-CoV-2 among three primer-probe sets in two different master mixes, Invitrogen™ SuperScript™ III One-Step RT-PCR and LightCycler Multiplex RNA Virus Master.
Results: The assay with TIB-Molbiol, IDT, and Phu Sa sets for LightCycler Multiplex RNA Virus Master or Invitrogen™ SuperScript™ III One-Step RT-PCR showed positive results from a single reaction of triplicate in the three days of 4.8 copies per reaction. R squared and amplification efficiency were 0.97 and ranged from 107 to 108%, respectively.
Conclusions: Our findings indicated that TIB-Molbiol, IDT, and Phu Sa primer-probe sets could be beneficial for the laboratory screening of SARS-CoV-2 by RT-qPCR assay of E gene. There is a need to consider the combination of these reagent sets as a new strategy to increase the testing capacity of screening programs for COVID-19.
Competing Interests: The authors have no conflicts of interest.
(Copyright © 2020 Hoang Quoc Cuong et al.)
Databáze: MEDLINE
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