Anti-inflammatory effects of dexmedetomidine on human amnion-derived WISH cells.

Autor: Shin SH; Department of Oral and Maxillofacial Surgery, School of Dentistry, Pusan National University, Yangsan, Korea., You JC; Department of Oral and Maxillofacial Surgery, School of Dentistry, Pusan National University, Yangsan, Korea., Ahn JH; Department of Dental Anesthesia and Pain Medicine, School of Dentistry, Pusan National University, Dental Research Institute, Yangsan, Korea., Kim YH; Department of Integrated Biological Science, Pusan National University, Busan 46241, Korea., Yoon JU; Department of Anesthesia and Pain Medicine, School of Medicine, Pusan National University, Yangsan, Korea., Cho AR; Department of Anesthesia and Pain Medicine, Pusan National University, School of Medicine, Yangsan, Republic of Korea., Kim EJ; Department of Dental Anesthesia and Pain Medicine, School of Dentistry, Pusan National University, Dental Research Institute, Yangsan, Korea.
Jazyk: angličtina
Zdroj: International journal of medical sciences [Int J Med Sci] 2020 Sep 09; Vol. 17 (16), pp. 2496-2504. Date of Electronic Publication: 2020 Sep 09 (Print Publication: 2020).
DOI: 10.7150/ijms.49909
Abstrakt: Background: To maintain the normal pregnancy, suppression of inflammatory signaling pathway is a crucial physiologic response. Dexmedetomidine has been used for labor analgesia or supplement of inadequate regional analgesia during delivery. And it has been reported that dexmedetomidine has an anti-inflammatory effect. In this study, we examined the influence of dexmedetomidine on the expression of cyclooxygenase-2 (COX-2), prostaglandin E 2 (PGE 2 ) and inflammatory cytokines in lipopolysaccharide (LPS)-stimulated human amnion-derived WISH cells. In addition, we evaluated the association of mitogen-activated protein kinase (MAPK) and nuclear factor kappa B (NF-κB) pathway in anti-inflammatory effect of dexmedetomidine. Methods: Human amnion-derived WISH cells were pretreated with various concentrations of dexmedetomidine (0.001-1 µg/ml) for 1 h and after then treated with LPS (1 µg/ml) for 24 h. MTT assay was conducted to evaluate the cytotoxicity. Nitric oxide (NO) production was analyzed using Griess-reaction microassay. RT-PCR was performed for analysis of mRNA expressions of COX-2, PGE 2 , tumor necrosis factor (TNF)-α and interlukin (IL)-1β. Protein expressions of COX-2, PGE 2 , p38 and NF-κB were analyzed by western blotting. Results: LPS and dexmedetomidine had no cytotoxic effect on WISH cells. There was no difference in NO production after dexmedetomidine pretreatment. The mRNA and protein expressions of COX-2 and PGE 2 were decreased by dexmedetomidine pretreatment in LPS-treated WISH cells. Dexmedetomidine also attenuated the LPS-induced mRNA expression of TNF-α and IL-1β. The activation of p38 and NF-κB was suppressed by dexmedetomidine pretreatment in LPS-treated WISH cells. Conclusion: We demonstrated that dexmedetomidine pretreatment suppressed the expressions of inflammatory mediators increased by LPS. In addition, this study suggests that anti-inflammatory effect of dexmedetomidine on WISH cells was mediated by the inhibitions of p38 and NF-κB activation.
Competing Interests: Competing Interests: The authors have declared that no competing interest exists.
(© The author(s).)
Databáze: MEDLINE
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