Surface area-to-volume ratio, not cellular viscoelasticity, is the major determinant of red blood cell traversal through small channels.

Autor: Namvar A; Department of Biochemistry and Molecular Biology, Bio21 Institute, University of Melbourne, Parkville, Victoria, Australia.; Department of Biomedical Engineering, University of Melbourne, Parkville, Victoria, Australia., Blanch AJ; Department of Biochemistry and Molecular Biology, Bio21 Institute, University of Melbourne, Parkville, Victoria, Australia., Dixon MW; Department of Biochemistry and Molecular Biology, Bio21 Institute, University of Melbourne, Parkville, Victoria, Australia., Carmo OMS; Department of Biochemistry and Molecular Biology, Bio21 Institute, University of Melbourne, Parkville, Victoria, Australia., Liu B; Department of Biochemistry and Molecular Biology, Bio21 Institute, University of Melbourne, Parkville, Victoria, Australia., Tiash S; Department of Biochemistry and Molecular Biology, Bio21 Institute, University of Melbourne, Parkville, Victoria, Australia., Looker O; Department of Biochemistry and Molecular Biology, Bio21 Institute, University of Melbourne, Parkville, Victoria, Australia., Andrew D; Department of Biochemistry and Molecular Biology, Bio21 Institute, University of Melbourne, Parkville, Victoria, Australia., Chan LJ; Division of Infection & Immunity, Walter & Eliza Hall Institute, Parkville, Victoria, Australia.; Department of Medical Biology, University of Melbourne, Parkville, Victoria, Australia., Tham WH; Division of Infection & Immunity, Walter & Eliza Hall Institute, Parkville, Victoria, Australia.; Department of Medical Biology, University of Melbourne, Parkville, Victoria, Australia., Lee PVS; Department of Biomedical Engineering, University of Melbourne, Parkville, Victoria, Australia., Rajagopal V; Department of Biomedical Engineering, University of Melbourne, Parkville, Victoria, Australia., Tilley L; Department of Biochemistry and Molecular Biology, Bio21 Institute, University of Melbourne, Parkville, Victoria, Australia.
Jazyk: angličtina
Zdroj: Cellular microbiology [Cell Microbiol] 2021 Jan; Vol. 23 (1), pp. e13270. Date of Electronic Publication: 2020 Oct 07.
DOI: 10.1111/cmi.13270
Abstrakt: The remarkable deformability of red blood cells (RBCs) depends on the viscoelasticity of the plasma membrane and cell contents and the surface area to volume (SA:V) ratio; however, it remains unclear which of these factors is the key determinant for passage through small capillaries. We used a microfluidic device to examine the traversal of normal, stiffened, swollen, parasitised and immature RBCs. We show that dramatic stiffening of RBCs had no measurable effect on their ability to traverse small channels. By contrast, a moderate decrease in the SA:V ratio had a marked effect on the equivalent cylinder diameter that is traversable by RBCs of similar cellular viscoelasticity. We developed a finite element model that provides a coherent rationale for the experimental observations, based on the nonlinear mechanical behaviour of the RBC membrane skeleton. We conclude that the SA:V ratio should be given more prominence in studies of RBC pathologies.
(© 2020 The Authors. Cellular Microbiology published by John Wiley & Sons Ltd.)
Databáze: MEDLINE
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