| Autor: |
Gamaleldin NM; Department of Microbiology, Faculty of Pharmacy, The British University in Egypt (BUE), Cairo 11837, Egypt.; Center for Drug Research and Development, Faculty of Pharmacy, The British University in Egypt (BUE), Cairo 11837, Egypt., Bakeer W; Department of Microbiology, Faculty of Pharmacy, Beni-Suef University, Beni-Suef 62514, Egypt., Sayed AM; Department of Pharmacognosy, Faculty of Pharmacy, Nahda University, Beni-Suef 62513, Egypt., Shamikh YI; Department of Microbiology & Immunology, Faculty of Pharmacy, Nahda University, Beni-Suef 62513, Egypt.; Virology Department, Egyptian Center for Research and Regenerative Medicine (ECRRM), Cairo 11517, Egypt., El-Gendy AO; Department of Microbiology, Faculty of Pharmacy, Beni-Suef University, Beni-Suef 62514, Egypt., Hassan HM; Department of Pharmacognosy, Faculty of Pharmacy, Beni-Suef University, Beni-Suef 62514, Egypt., Horn H; Independent Researcher, 69126 Heidelberg, Germany., Abdelmohsen UR; Department of Pharmacognosy, Faculty of Pharmacy, Minia University, Minia 61519, Egypt.; Department of Pharmacognosy, Faculty of Pharmacy, Deraya University, New Minia 61111, Egypt., Hozzein WN; Bioproducts Research Chair, Zoology Department, College of Science, King Saud University, Riyadh 11451, Saudi Arabia.; Botany and Microbiology Department, Faculty of Science, Beni-Suef University, Beni-Suef 62512, Egypt. |
| Abstrakt: |
In the present study, we investigated the actinomycetes associated with the Red Sea-derived soft coral Sarcophyton glaucum in terms of biological and chemical diversity. Three strains were cultivated and identified to be members of genera Micromonospora , Streptomyces , and Nocardiopsis ; out of them, Micromonospora sp. UR17 was putatively characterized as a new species. In order to explore the chemical diversity of these actinobacteria as far as possible, they were subjected to a series of fermentation experiments under altering conditions, that is, solid and liquid fermentation along with co-fermentation with a mycolic acid-containing strain, namely Nocardia sp. UR23. Each treatment was found to affect these actinomycetes differently in terms of biological activity (i.e., antitrypanosomal activity) and chemical profiles evidenced by LC-HRES-MS-based metabolomics and multivariate analysis. Thereafter, orthogonal projections to latent structures discriminant analysis (OPLS-DA) suggested a number of metabolites to be associated with the antitrypanosomal activity of the active extracts. The subsequent in silico screenings (neural networking-based and docking-based) further supported the OPLS-DA results and prioritized desferrioxamine B ( 3 ), bafilomycin D ( 10 ), and bafilomycin A1 ( 11 ) as possible antitrypanosomal agents. Our approach in this study can be applied as a primary step in the exploration of bioactive natural products, particularly those from actinomycetes. |
