Origanum vulgare L. leaves extract alleviates testis and sperm damages induced by finasteride: Biochemical, Immunohistological and apoptosis genes based evidences.

Autor: Chen X; Shaanxi Institute of Zoology, Xi'an, China.; College of Life Sciences, Shaanxi Normal University, Xi'an, China., Feng H; Shaanxi Institute of Zoology, Xi'an, China., El-Kott AF; Biology Department, Faculty of Science, King Khalid University, Abha, Saudi Arabia.; Zoology Department, College of Science, Damanhour University, Damanhour, Egypt., Abd-Ella EM; Zoology Department, College of Science, Fayoum University, Fayoum, Egypt.; Biology Department, College of Science and Art, Al-Baha University, Al-Mandaq, Saudi Arabia.
Jazyk: angličtina
Zdroj: Andrologia [Andrologia] 2020 Dec; Vol. 52 (11), pp. e13823. Date of Electronic Publication: 2020 Sep 23.
DOI: 10.1111/and.13823
Abstrakt: The aim of the current study was to investigate antioxidant, anti-inflammatory and anti-apoptotic effects of Origanum vulgare on finasteride-induced oxidative injury in mouse testis and sperm parameters. Thirty BALB/c mice were divided into 5 groups: negative control, received 0.5 ml/day distilled water; positive control, received 25 mg/kg finasteride orally; and three groups received 100, 200 and 400 mg/kg/day O. vulgare extract plus 25 mg kg -1  day -1 finasteride for 35 days. At day 36, serum luteinising hormone, follicle-stimulating hormone and testosterone, inflammatory cytokines (IL-6, TNF-α, IL-1β), glutathione peroxidase, superoxide dismutase and nitric oxide levels were assessed. Also, apoptotic changes investigated through genes expression and immunohistochemical staining. Finasteride in 35 days resulted in significant destructive alterations in the testis architecture, suppressed antioxidant enzymes and increased lipid peroxidation. The expression of Bcl-2 was down-regulated, whereas p53 and caspase-3 were up-regulated. Origanum vulgare improved the serum level of hormones and restored the antioxidant defence. 200 and 400 mg/kg/day of O. vulgare alleviated the testis structure and sperm parameters, up-regulated the anti-apoptotic gene Bcl-2 and down-regulated the p53, caspase-3 genes in treated groups. The findings indicate that O. vulgare extract improved function and structure of testis tissue against finasteride-induced testicular toxicity.
(© 2020 Wiley-VCH GmbH.)
Databáze: MEDLINE
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