Methods for Automated Single Cell Isolation and Sub-Cloning of Human Pluripotent Stem Cells.

Autor: Vallone VF; Charité-Universitätsmedizin Berlin, Berlin, Germany.; Berlin Institute of Health (BIH), BIH Stem Cell Core Facility, Berlin, Germany., Telugu NS; Max Delbrück Center for Molecular Medicine in the Helmholtz Association (MDC), Berlin, Germany.; BIH Stem Cell Core Facility, Berlin Institute of Health (BIH), Berlin, Germany., Fischer I; Charité-Universitätsmedizin Berlin, Berlin, Germany.; Berlin Institute of Health (BIH), BIH Stem Cell Core Facility, Berlin, Germany., Miller D; Max Delbrück Center for Molecular Medicine in the Helmholtz Association (MDC), Berlin, Germany.; BIH Stem Cell Core Facility, Berlin Institute of Health (BIH), Berlin, Germany.; DZHK (German Centre for Cardiovascular Research), partner site Berlin, Berlin, Germany., Schommer S; Max Delbrück Center for Molecular Medicine in the Helmholtz Association (MDC), Berlin, Germany.; BIH Stem Cell Core Facility, Berlin Institute of Health (BIH), Berlin, Germany., Diecke S; Max Delbrück Center for Molecular Medicine in the Helmholtz Association (MDC), Berlin, Germany.; BIH Stem Cell Core Facility, Berlin Institute of Health (BIH), Berlin, Germany.; DZHK (German Centre for Cardiovascular Research), partner site Berlin, Berlin, Germany., Stachelscheid H; Charité-Universitätsmedizin Berlin, Berlin, Germany.; Berlin Institute of Health (BIH), BIH Stem Cell Core Facility, Berlin, Germany.
Jazyk: angličtina
Zdroj: Current protocols in stem cell biology [Curr Protoc Stem Cell Biol] 2020 Dec; Vol. 55 (1), pp. e123.
DOI: 10.1002/cpsc.123
Abstrakt: Advances in human pluripotent stem cell (hPSC) techniques have led them to become a widely used and powerful tool for a vast array of applications, including disease modeling, developmental studies, drug discovery and testing, and emerging cell-based therapies. hPSC workflows that require clonal expansion from single cells, such as CRISPR/Cas9-mediated genome editing, face major challenges in terms of efficiency, cost, and precision. Classical sub-cloning approaches depend on limiting dilution and manual colony picking, which are both time-consuming and labor-intensive, and lack a real proof of clonality. Here we describe the application of three different automated cell isolation and dispensing devices that can enhance the single-cell cloning process for hPSCs. In combination with optimized cell culture conditions, these devices offer an attractive alternative compared to manual methods. We explore various aspects of each device system and define protocols for their practical application. Following the workflow described here, single cell-derived hPSC sub-clones from each system maintain pluripotency and genetic stability. Furthermore, the workflows can be applied to uncover karyotypic mosaicism prevalent in bulk hPSC cultures. Our robust automated workflow facilitates high-throughput hPSC clonal selection and expansion, urgently needed in the operational pipelines of hPSC applications. © 2020 The Authors. Basic Protocol: Efficient automated hPSC single cell seeding and clonal expansion using the iotaSciences IsoCell platform Alternate Protocol 1: hPSC single cell seeding and clonal expansion using the Cellenion CellenONE single-cell dispenser Alternate Protocol 2: hPSC single cell seeding and clonal expansion using the Cytena single-cell dispenser Support Protocol 1: Coating cell culture plates with Geltrex Support Protocol 2: hPSC maintenance in defined feeder-free conditions Support Protocol 3: hPSC passaging in clumps Support Protocol 4: Laminin 521 coating of IsoCell plates and 96-well/384-well plates Support Protocol 5: Preparation of medium containing anti-apoptotic small molecules Support Protocol 6: 96- and 384-well target plate preparation prior to single cell seeding Support Protocol 7: Single cell dissociation of hPSCs Support Protocol 8: IsoCell-, CellenONE-, and Cytena-derived hPSC clone subculture and expansion.
(© 2020 The Authors.)
Databáze: MEDLINE
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