Hypoxia and hypocapnia storage of γ-irradiated red cell concentrates.

Autor: Bardyn M; Laboratoire de Recherche surles Produits Sanguins, Recherche et Développement Produits, Transfusion Interrégionale CRS, Epalinges, Switzerland., Crettaz D; Laboratoire de Recherche surles Produits Sanguins, Recherche et Développement Produits, Transfusion Interrégionale CRS, Epalinges, Switzerland., Borlet M; Laboratoire de Recherche surles Produits Sanguins, Recherche et Développement Produits, Transfusion Interrégionale CRS, Epalinges, Switzerland., Längst E; Laboratoire de Recherche surles Produits Sanguins, Recherche et Développement Produits, Transfusion Interrégionale CRS, Epalinges, Switzerland.; Centre de Transfusion Sanguine, Faculté de Biologie et de Médecine, University of Lausanne, Lausanne, Switzerland., Martin A; Laboratoire de Préparation Cellulaire et d'Analyses, Recherche et Développement Produits, Transfusion Interrégionale CRS, Epalinges, Switzerland., Abonnenc M; Laboratoire de Recherche surles Produits Sanguins, Recherche et Développement Produits, Transfusion Interrégionale CRS, Epalinges, Switzerland.; Laboratoire de Préparation Cellulaire et d'Analyses, Recherche et Développement Produits, Transfusion Interrégionale CRS, Epalinges, Switzerland., Tissot JD; Laboratoire de Recherche surles Produits Sanguins, Recherche et Développement Produits, Transfusion Interrégionale CRS, Epalinges, Switzerland.; Centre de Transfusion Sanguine, Faculté de Biologie et de Médecine, University of Lausanne, Lausanne, Switzerland., Dunham A; Hemanext, Lexington, MA, USA., Yoshida T; Hemanext, Lexington, MA, USA., Prudent M; Laboratoire de Recherche surles Produits Sanguins, Recherche et Développement Produits, Transfusion Interrégionale CRS, Epalinges, Switzerland.; Centre de Transfusion Sanguine, Faculté de Biologie et de Médecine, University of Lausanne, Lausanne, Switzerland.
Jazyk: angličtina
Zdroj: Blood transfusion = Trasfusione del sangue [Blood Transfus] 2021 Jul; Vol. 19 (4), pp. 300-308. Date of Electronic Publication: 2020 Sep 17.
DOI: 10.2450/2020.0075-20
Abstrakt: Background: γ-irradiation is used to treat red blood cell (RBC) concentrates (RCCs) transfused to immunosuppressed patients. This treatment damages RBCs and increases storage lesions. Several studies have shown the beneficial effect of reducing O 2 content during RBC storage. The present research work investigated the effect of γ-irradiation on RCCs stored under normal and hypoxia/hypocapnia conditions.
Materials and Methods: O 2 concentration (measured as oxyhaemoglobin fraction, sO 2 ) and ABO-matched RCCs from whole blood donations, leukoreduced and prepared in phosphate, adenine, glucose, guanosine, saline and mannitol (PAGGSM) were pooled and split in two identical RCCs within 24 h post donation. One bag (Hx) was submitted to O 2 and CO 2 adsorption for 3 h on an orbital shaker at 22±2 °C and then transferred to a storage bag impermeable to gas. The other bag (Ctrl) was left as it was. The two bags were then stored at 4 °C. γ-irradiation (25 Gy) was applied at day 2 or 14, and the RCCs were stored until day 43. Different parameters (metabolites, haemolysis, morphology) were measured.
Results: Starting sO 2 values were 63.7±18.4% (n=12) in Ctrl and 20.8±9.8% (n=12) in Hx bags, and reached 90.8±9.1% and 6.6±5.9% at day 43, respectively. As expected, an increase in glycolysis rate was observed after deoxygenation. Extracellular potassium concentrations were identical and reached around 70 mM at expiry with an irradiation-dependent kinetic release. No difference in haemolysis was observed after irradiation on day 2 in either group (<0.40%, p>0.9999). When irradiated at day 14, haemolysis was lower (p=0.033) in RCCs under hypoxia at the end of storage (day 28, 0.67±0.16%) compared to control (1.06±0.33%). Percentages of spherocytes were lower under hypoxia.
Discussion: The storage under hypoxia provided equivalent storage when RCCs were irradiated at day 2 and was advantageous when irradiated at day 14. In summary, O 2 -depletion of RCCs enable a better storage of RBCs, particularly when late irradiation is applied.
Databáze: MEDLINE
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