qRT-PCR Platforms for Diagnosing and Reporting SARS-CoV-2 Infection in Human Samples.

Autor:	Giadone RM; Center for Regenerative Medicine of Boston University and Boston Medical Center, Boston, MA 02118, USA., Mithal A; Center for Regenerative Medicine of Boston University and Boston Medical Center, Boston, MA 02118, USA., Miller GJ; Center for Regenerative Medicine of Boston University and Boston Medical Center, Boston, MA 02118, USA., Matte TM; Center for Regenerative Medicine of Boston University and Boston Medical Center, Boston, MA 02118, USA., Yeung AK; Center for Regenerative Medicine of Boston University and Boston Medical Center, Boston, MA 02118, USA., Dowrey TW; Center for Regenerative Medicine of Boston University and Boston Medical Center, Boston, MA 02118, USA., Werder RB; Center for Regenerative Medicine of Boston University and Boston Medical Center, Boston, MA 02118, USA., Miller NS; Department of Pathology and Laboratory Medicine, Boston Medical Center and Boston University School of Medicine, Boston, MA 02218, USA., Andry CD; Department of Pathology and Laboratory Medicine, Boston Medical Center and Boston University School of Medicine, Boston, MA 02218, USA., Vanuytsel K; Center for Regenerative Medicine of Boston University and Boston Medical Center, Boston, MA 02118, USA.; Section of Hematology and Oncology, Department of Medicine, Boston University School of Medicine, Boston, MA 02218, USA., Murphy GJ; Center for Regenerative Medicine of Boston University and Boston Medical Center, Boston, MA 02118, USA.; Section of Hematology and Oncology, Department of Medicine, Boston University School of Medicine, Boston, MA 02218, USA.
Jazyk:	angličtina
Zdroj:	STAR protocols [STAR Protoc] 2020 Sep 18; Vol. 1 (2), pp. 100102. Date of Electronic Publication: 2020 Sep 15.
DOI:	10.1016/j.xpro.2020.100102
Abstrakt:	The protocols herein outline the use of qRT-PCR to detect the presence of SARS-CoV-2 genomic RNA in patient samples. In order to cope with potential fluctuations in supply chain and testing demands and to enable expedient adaptation of reagents and assays on hand, we include details for three parallel methodologies (one- and two-step singleplex and one-step multiplex assays). The diagnostic platforms described can be easily adapted by basic science research laboratories for SARS-CoV-2 diagnostic testing with relatively short turnaround time. For complete details on the use and execution of this protocol, please refer to Vanuytsel et al. (2020). Competing Interests: The authors declare no competing interests. (© 2020 The Author(s).)
Databáze:	MEDLINE
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