Expanding sacrificially printed microfluidic channel-embedded paper devices for construction of volumetric tissue models in vitro.

Autor:	Li H; Division of Engineering in Medicine, Brigham and Women's Hospital, Department of Medicine, Harvard Medical School, Cambridge, MA 02139, United States of America.; College of Light Industry and Textile, Qiqihar University, Qiqihar, Heilongjiang 161000, People's Republic of China.; MIIT Key Laboratory of Critical Materials Technology for New Energy Conversion and Storage, School of Chemistry and Chemical Engineering, Harbin Institute of Technology, Harbin, Heilongjiang 150001, People's Republic of China., Cheng F; Division of Engineering in Medicine, Brigham and Women's Hospital, Department of Medicine, Harvard Medical School, Cambridge, MA 02139, United States of America.; MIIT Key Laboratory of Critical Materials Technology for New Energy Conversion and Storage, School of Chemistry and Chemical Engineering, Harbin Institute of Technology, Harbin, Heilongjiang 150001, People's Republic of China., Li W; Division of Engineering in Medicine, Brigham and Women's Hospital, Department of Medicine, Harvard Medical School, Cambridge, MA 02139, United States of America., Cao X; Division of Engineering in Medicine, Brigham and Women's Hospital, Department of Medicine, Harvard Medical School, Cambridge, MA 02139, United States of America., Wang Z; Division of Engineering in Medicine, Brigham and Women's Hospital, Department of Medicine, Harvard Medical School, Cambridge, MA 02139, United States of America., Wang M; Division of Engineering in Medicine, Brigham and Women's Hospital, Department of Medicine, Harvard Medical School, Cambridge, MA 02139, United States of America., Robledo-Lara JA; Division of Engineering in Medicine, Brigham and Women's Hospital, Department of Medicine, Harvard Medical School, Cambridge, MA 02139, United States of America.; Departamento de Ingenieriéa Mecatroénica y Eleéctrica, Escuela de Ingenieriéa y Ciencias, Tecnoloégico de Monterrey, CP78211, San Luis Potosí, San Luis Potosí, Mexico., Liao J; Division of Engineering in Medicine, Brigham and Women's Hospital, Department of Medicine, Harvard Medical School, Cambridge, MA 02139, United States of America., Chávez-Madero C; Division of Engineering in Medicine, Brigham and Women's Hospital, Department of Medicine, Harvard Medical School, Cambridge, MA 02139, United States of America.; Centro de Biotecnología-FEMSA, Tecnologico de Monterrey, Monterrey CP 64849, Mexico., Hassan S; Division of Engineering in Medicine, Brigham and Women's Hospital, Department of Medicine, Harvard Medical School, Cambridge, MA 02139, United States of America., Xie J; Holland Regenerative Medicine Program, University of Nebraska Medical Center, Omaha, NE 68198, United States of America., Trujillo-de Santiago G; Centro de Biotecnología-FEMSA, Tecnologico de Monterrey, Monterrey CP 64849, Mexico., Álvarez MM; Centro de Biotecnología-FEMSA, Tecnologico de Monterrey, Monterrey CP 64849, Mexico., He J; MIIT Key Laboratory of Critical Materials Technology for New Energy Conversion and Storage, School of Chemistry and Chemical Engineering, Harbin Institute of Technology, Harbin, Heilongjiang 150001, People's Republic of China., Zhang YS; Division of Engineering in Medicine, Brigham and Women's Hospital, Department of Medicine, Harvard Medical School, Cambridge, MA 02139, United States of America.
Jazyk:	angličtina
Zdroj:	Biofabrication [Biofabrication] 2020 Sep 18; Vol. 12 (4), pp. 045027. Date of Electronic Publication: 2020 Sep 18.
DOI:	10.1088/1758-5090/abb11e
Abstrakt:	We report a method for expanding microchannel-embedded paper devices using a precisely controlled gas-foaming technique for the generation of volumetric tissue models in vitro. We successfully fabricated hollow, perfusable microchannel patterns contained in a densely entangled network of bacterial cellulose nanofibrils using matrix-assisted sacrificial three-dimensional printing, and demonstrated the maintenance of their structural integrity after gas-foaming-enabled expansion in an aqueous solution of NaBH 4 . The resulting expanded microchannel-embedded paper devices showed multilayered laminar structures with controllable thicknesses as a function of both NaBH 4 concentration and expansion time. With expansion, the thickness and porosity of the bacterial cellulose network were significantly increased. As such, cellular infiltration was promoted comparing to as-prepared, non-expanded devices. This simple technique enables the generation of truly volumetric, cost-effective human-based tissue models, such as vascularized tumor models, for potential applications in preclinical drug screening and personalized therapeutic selection.
Databáze:	MEDLINE
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