Longitudinal study on oral shedding of human betaherpesviruses 6 and 7 in renal transplant recipients reveals active replication.

Autor: Raposo JV; Instituto Oswaldo Cruz - IOC/FIOCRUZ - Fundação Oswaldo Cruz, Rio De Janeiro, Brasil., Sarmento DJS; Departamento de Estomatologia, Faculdade de Odontologia da Universidade de São Paulo - FOUSP, São Paulo, SP, Brasil., Pinto RBDS; Instituto Oswaldo Cruz - IOC/FIOCRUZ - Fundação Oswaldo Cruz, Rio De Janeiro, Brasil., Lopes AO; Instituto Oswaldo Cruz - IOC/FIOCRUZ - Fundação Oswaldo Cruz, Rio De Janeiro, Brasil., Gallottini M; Departamento de Estomatologia, Faculdade de Odontologia da Universidade de São Paulo - FOUSP, São Paulo, SP, Brasil., Tozetto-Mendoza TR; Laboratório de Virologia, Instituto de Medicina Tropical de São Paulo - IMTSP - Faculdade de Medicina da Universidade de São Paulo, São Paulo, SP, Brasil., Braz-Silva PH; Departamento de Estomatologia, Faculdade de Odontologia da Universidade de São Paulo - FOUSP, São Paulo, SP, Brasil.; Laboratório de Virologia, Instituto de Medicina Tropical de São Paulo - IMTSP - Faculdade de Medicina da Universidade de São Paulo, São Paulo, SP, Brasil., de Paula VS; Instituto Oswaldo Cruz - IOC/FIOCRUZ - Fundação Oswaldo Cruz, Rio De Janeiro, Brasil.
Jazyk: angličtina
Zdroj: Journal of oral microbiology [J Oral Microbiol] 2020 Jun 30; Vol. 12 (1), pp. 1785801. Date of Electronic Publication: 2020 Jun 30.
DOI: 10.1080/20002297.2020.1785801
Abstrakt: Backgroung: Roseolovirus latency and persistence in salivary glands that are frequently reactivated after renal transplantation to cause infection have been reported. However, limited information is available on the persistence and excretion of HHV-6 and HHV-7 during and after transplant.
Methods: 32 renal transplant recipients were followed up before (T1) and after transplant (T2 and T3) and viral replication (via assessment of mRNA) in oral fluid samples investigated. Roseolovirus DNA was detected and quantified via multiplex qPCR. For evaluation of mRNA replication, positive samples were subjected to nested RT-PCR.
Results: Viral replication of HHV-7 was significantly increased during T3 (72.9%), compared to the pre-transplant period T1 (25%; McNemar Test, p= 0.001). Analysis of the viral replicative to quantitative ratio disclosed ahigher number of DNA copies (>10 6 ) in positive cases of replication (p < 0.001). Astrong positive correlation (Spearman correlation coefficient = 0.781; p< 0.001) was evident between viral quantities of Roseoloviruses.
Conclusion: Our findings consistently suggest that the salivary gland is an important site of active and persistent infection by roseoloviruses. In view of the increasing problem of Roseoloviruses, pre- and post-transplantation, viral surveillance and monitoring of active replication are pivotal steps for effective screening and treatment of renal transplant patients.
Competing Interests: No reported conflicts. Conflicts that the editors consider relevant to the content of the manuscript have been disclosed.
(© 2020 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.)
Databáze: MEDLINE
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