| Autor: |
Pinheiro-Lima B; Embrapa Recursos Genéticos e Biotecnologia, Brasília DF 70770-017, Brazil.; Departamento de Fitopatologia, Instituto de Biologia, Universidade de Brasília, Brasília DF 70275-970, Brazil.; Departamento de Biologia Celular, Instituto de Biologia, Universidade de Brasília, Brasília DF 70275-970, Brazil., Pereira-Carvalho RC; Departamento de Fitopatologia, Instituto de Biologia, Universidade de Brasília, Brasília DF 70275-970, Brazil., Alves-Freitas DMT; Embrapa Recursos Genéticos e Biotecnologia, Brasília DF 70770-017, Brazil., Kitajima EW; Departamento de Fitopatologia, Escola Superior de Agricultura Luiz de Queiroz, Piracicaba SP 13418-900, Brazil., Vidal AH; Embrapa Recursos Genéticos e Biotecnologia, Brasília DF 70770-017, Brazil.; Departamento de Biologia Celular, Instituto de Biologia, Universidade de Brasília, Brasília DF 70275-970, Brazil., Lacorte C; Embrapa Recursos Genéticos e Biotecnologia, Brasília DF 70770-017, Brazil., Godinho MT; Embrapa Recursos Genéticos e Biotecnologia, Brasília DF 70770-017, Brazil., Fontenele RS; The Biodesign Center for Fundamental and Applied Microbiomics, Center for Evolution and Medicine School of Life Sciences, Arizona State University, Tempe, AZ 85287-5001, USA., Faria JC; Embrapa Arroz e Feijão, Goiânia GO 75375-000, Brazil., Abreu EFM; Embrapa Recursos Genéticos e Biotecnologia, Brasília DF 70770-017, Brazil., Varsani A; The Biodesign Center for Fundamental and Applied Microbiomics, Center for Evolution and Medicine School of Life Sciences, Arizona State University, Tempe, AZ 85287-5001, USA.; Structural Biology Research Unit, Department of Integrative Biomedical Sciences, University of Cape Town, Observatory, Cape Town 7701, South Africa., Ribeiro SG; Embrapa Recursos Genéticos e Biotecnologia, Brasília DF 70770-017, Brazil., Melo FL; Departamento de Fitopatologia, Instituto de Biologia, Universidade de Brasília, Brasília DF 70275-970, Brazil.; Departamento de Biologia Celular, Instituto de Biologia, Universidade de Brasília, Brasília DF 70275-970, Brazil. |
| Abstrakt: |
The knowledge of genomic data of new plant viruses is increasing exponentially; however, some aspects of their biology, such as vectors and host range, remain mostly unknown. This information is crucial for the understanding of virus-plant interactions, control strategies, and mechanisms to prevent outbreaks. Typically, rhabdoviruses infect monocot and dicot plants and are vectored in nature by hemipteran sap-sucking insects, including aphids, leafhoppers, and planthoppers. However, several strains of a potentially whitefly-transmitted virus, papaya cytorhabdovirus, were recently described: (i) bean-associated cytorhabdovirus (BaCV) in Brazil, (ii) papaya virus E (PpVE) in Ecuador, and (iii) citrus-associated rhabdovirus (CiaRV) in China. Here, we examine the potential of the Bemisia tabaci Middle East-Asia Minor 1 (MEAM1) to transmit BaCV, its morphological and cytopathological characteristics, and assess the incidence of BaCV across bean producing areas in Brazil. Our results show that BaCV is efficiently transmitted, in experimental conditions, by B. tabaci MEAM1 to bean cultivars, and with lower efficiency to cowpea and soybean. Moreover, we detected BaCV RNA in viruliferous whiteflies but we were unable to visualize viral particles or viroplasm in the whitefly tissues. BaCV could not be singly isolated for pathogenicity tests, identification of the induced symptoms, and the transmission assay. BaCV was detected in five out of the seven states in Brazil included in our study, suggesting that it is widely distributed throughout bean producing areas in the country. This is the first report of a whitefly-transmitted rhabdovirus. |
