Alu -repeat polymorphism in the tissue plasminogen activator ( t-PA ) gene, seminal t-PA concentration, and male fertility impairment: A case-control study.

Autor:	Tahtamouni LH; Department of Biology and Biotechnology, Faculty of Science, the Hashemite University, Zarqa, Jordan.; Department of Biochemistry and Molecular Biology, College of Natural Sciences, Colorado State University, Fort Collins, Colorado, USA., Hamdan MN; Department of Biology and Biotechnology, Faculty of Science, the Hashemite University, Zarqa, Jordan., Al-Mazaydeh ZA; Department of Biology and Biotechnology, Faculty of Science, the Hashemite University, Zarqa, Jordan., Bawadi RM; Department of Physiology and Biochemistry, School of Medicine, the University of Jordan, Amman, Jordan., Rammaha MS; Department of Biology and Biotechnology, Faculty of Science, the Hashemite University, Zarqa, Jordan., Zghoul AM; Department of Biology and Biotechnology, Faculty of Science, the Hashemite University, Zarqa, Jordan., Ahram MA; Department of Physiology and Biochemistry, School of Medicine, the University of Jordan, Amman, Jordan., Yasin SR; Department of Biology and Biotechnology, Faculty of Science, the Hashemite University, Zarqa, Jordan.
Jazyk:	angličtina
Zdroj:	International journal of reproductive biomedicine [Int J Reprod Biomed] 2020 Aug 19; Vol. 18 (8), pp. 571-578. Date of Electronic Publication: 2020 Aug 19 (Print Publication: 2020).
DOI:	10.18502/ijrm.v13i8.7496
Abstrakt:	Background: Tissue plasminogen activator (t-PA) is a protein involved in the fibrinolytic system that catalyzes the conversion of plasminogen into the active plasmin. The activity of t-PA is controlled by plasminogen activator inhibitor-1. t-PA has crucial functions during spermatogenesis. One polymorphism was reported for t-PA gene, either the presence of a 300-bp Alu -repeat ( Alu + ) or its absence ( Alu - ). Objective: The current work aimed at studying the association between Alu polymorphism in the t-PA gene and male infertility. Materials and Methods: Using polymerase chain reaction on genomic DNA isolated from the blood of 79 participants, a region polymorphic for Alu element insertion in t-PA gene was amplified. In addition, total t-PA concentration, plasminogen activator inhibitor-1 /t-PA complex concentration, and t-PA activity in seminal plasma were measured by enzyme-linked immunosorbent assay. Results: The results indicate that the percentage of infertile participants (n = 50) who were homozygous for t-PA Alu insertion ( Alu + / + ), heterozygous Alu + / - or homozygous for t-PA Alu deletion ( Alu - / - ) did not change significantly (p = 0.43, 0.81, and 0.85, respectively) when compared with the control participants (n = 29). On the other hand, a significant decrease (p = 0.0001) of t-PA total concentration in seminal plasma was observed in the infertile group in comparison with the control group. However, the results indicate that there is no association between the t-PA Alu different genotypes and the total t-PA seminal concentration in the infertile group when compared to the control group (p = 0.63). Conclusion: Data obtained from the current study does not support an association between t-PA Alu polymorphism and t-PA seminal concentration or male infertility. Competing Interests: The authors declare that there is no conflict of interest. (Copyright © 2020 Tahtamouni et al.)
Databáze:	MEDLINE
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