Triterpenoids and steroids isolated from Anatolian Capparis ovata and their activity on the expression of inflammatory cytokines.
| Autor: | Gazioglu I; Department of Analytical Chemistry, Faculty of Pharmacy, Bezmialem Vakif University, Istanbul, Turkey., Semen S; Forensic Toxicology Laboratories, Institute of Forensic Sciences, Istanbul-Cerrahpasa University, Istanbul, Turkey., Acar OO; Department of Biology, Faculty of Arts and Sciences, Pamukkale University, Denizli, Turkey., Kolak U; Department of Analytical Chemistry, Faculty of Pharmacy, Istanbul University, Istanbul, Turkey., Sen A; Department of Biology, Faculty of Arts and Sciences, Pamukkale University, Denizli, Turkey.; Department of Molecular Biology and Genetics, Faculty of Life & Natural Sciences, Abdullah Gul University, Kayseri, Turkey., Topcu G; Department of Pharmacognosy and Phytochemistry, Faculty of Pharmacy, Bezmialem Vakif University, Istanbul, Turkey. |
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| Jazyk: | angličtina |
| Zdroj: | Pharmaceutical biology [Pharm Biol] 2020 Dec; Vol. 58 (1), pp. 925-931. |
| DOI: | 10.1080/13880209.2020.1814356 |
| Abstrakt: | Context: Capparis L. (Capparaceae) is grown worldwide. Caper has been used in traditional medicine to treat various diseases including rheumatism, kidney, liver, stomach, as well as headache and toothache. Objective: To isolate and elucidate of the secondary metabolites of the C. ovata extracts which are responsible for their anti-inflammatory activities. Materials and Methods: Buds, fruits, flowers, leaves and stems of C. ovata Desf. was dried, cut to pieces, then ground separately. From their dichloromethane/hexane (1:1) extracts, eight compounds were isolated and their structures were elucidated by NMR, mass spectroscopic techniques. The effects of compounds on the expression of inflammatory cytokines in SH-SY5Y cell lines were examined by qRT-PCR ranging from 4 to 96 µM. Cell viability was expressed as a percentage of the control, untreated cells. Results: This is a first report on isolation of triterpenoids and steroids from C. ovata with anti-inflammatory activity. One new triterpenoid ester olean-12-en-3β,28-diol, 3β-pentacosanoate ( 1 ) and two new natural steroids 5α,6α-epoxycholestan-3β-ol ( 5 ) and 5β,6β-epoxycholestan-3β-ol ( 6 ) were elucidated besides known compounds; oleanolic acid ( 2 ), ursolic acid ( 3 ), β-sitosterol ( 4 ), stigmast-5,22-dien-3β-myristate ( 7 ) and bismethyl-octylphthalate ( 8 ). mRNA expression levels as EC Discussion and Conclusions: Triterpenoids and steroids isolated from C. ovata were found to be moderate-strong anti-inflammatory compounds. Particularly, compounds 1 and 3 were found to be promising therapeutic agents in the treatment of inflammatory and autoimmune diseases. |
| Databáze: | MEDLINE |
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