| Autor: |
Bueno Duarte GH; ThoMSon Mass Spectrometry Laboratory, University of Campinas-UNICAMP, Campinas, SP, 13083-970, Brazil.; Laboratory of Analysis of Biomolecules Tiselius, University of Campinas-UNICAMP, Campinas, SP, 13083-970, Brazil., de Piloto Fernandes AMA; Health Sciences Postgraduate Program, São Francisco University-USF, Bragança Paulista, SP, 12916-900, Brazil., Silva AAR; Health Sciences Postgraduate Program, São Francisco University-USF, Bragança Paulista, SP, 12916-900, Brazil., Zamora-Obando HR; Laboratory of Analysis of Biomolecules Tiselius, University of Campinas-UNICAMP, Campinas, SP, 13083-970, Brazil., Amaral AG; Laboratory of Analysis of Biomolecules Tiselius, University of Campinas-UNICAMP, Campinas, SP, 13083-970, Brazil., de Sousa Mesquita A; Laboratory of Analysis of Biomolecules Tiselius, University of Campinas-UNICAMP, Campinas, SP, 13083-970, Brazil., Schmidt-Filho J; Department of Hematology, AC Camargo Cancer Center, São Paulo, SP, 01525-001, Brazil., Cordeiro de Lima VC; Department of Medical Oncology, AC Camargo Cancer Center, São Paulo, SP, 01525-001, Brazil., D'Almeida Costa F; Department of Pathology, AC Camargo Cancer Center, São Paulo, SP, 01509-010, Brazil., Andrade VP; Department of Pathology, AC Camargo Cancer Center, São Paulo, SP, 01509-010, Brazil., Porcari AM; Health Sciences Postgraduate Program, São Francisco University-USF, Bragança Paulista, SP, 12916-900, Brazil., Eberlin MN; MackMass Laboratory for Mass Spectrometry school of engineering - PPGEMN, Mackenzie Presbyterian University, São Paulo, SP, 01302-907, Brazil., Simionato AVC; Laboratory of Analysis of Biomolecules Tiselius, University of Campinas-UNICAMP, Campinas, SP, 13083-970, Brazil. avsimionato@unicamp.br.; National Institute of Science and Technology in Bioanalytics (INCTBio), Campinas, SP, 13083-970, Brazil. avsimionato@unicamp.br. |
| Abstrakt: |
Non-Hodgkin's lymphoma (NHL) is a cancer of the lymphatic system where the lymphoid and hematopoietic tissues are infiltrated by malignant neoplasms of B, T, and natural killer lymphocytes. Effective and less invasive methods for NHL screening are urgently needed. Herein, we report an untargeted gas chromatography-mass spectrometry (GC-MS) method to investigate metabolic changes in non-volatile derivatized compounds from urine samples of NHL patients (N = 15) and compare them to healthy controls (N = 34). Uni- and multivariate data analysis showed 18 endogenous metabolites, including amino acids and their metabolites, sugars, small organic acids, and vitamins, as statistically significant for group differentiation. A receiver operating characteristic curve (ROC) generated from a support vector machine (SVM) algorithm-based model achieved 0.998 of predictive accuracy, displaying the potential and relevance of GC-MS-detected urinary non-volatile compounds for predictive purposes. Furthermore, a specific panel of key metabolites was also evaluated, showing similar results. All in all, our results indicate that this robust GC-MS method is an effective screening tool for NHL diagnosis and it is able to highlight different pathways of the disease. Graphical Abstract. |
