| Autor: |
Jafari S; Pharmaceutical Sciences Research Center, Health Institute, Kermanshah University of Medical Sciences, Kermanshah, Iran., Izadi Z; Pharmaceutical Sciences Research Center, Health Institute, Kermanshah University of Medical Sciences, Kermanshah, Iran., Alaei L; Department of Biology and Biotechnology, Faculty of Sciences, University of Kurdistan, Sanandaj, Iran., Jaymand M; Nano Drug Delivery Research Center, Health Technology Institute, Kermanshah University of Medical Sciences, Kermanshah, Iran., Samadian H; Nano Drug Delivery Research Center, Health Technology Institute, Kermanshah University of Medical Sciences, Kermanshah, Iran., Kashani VO; Department of Physical Education, Faculty of Human Sciences, Semnan University, Semnan, Iran. vkashani@semnan.ac.ir., Derakhshankhah H; Pharmaceutical Sciences Research Center, Health Institute, Kermanshah University of Medical Sciences, Kermanshah, Iran. Derakhshankhah.hossein@gmail.com., Hayati P; Persian Gulf Science and Technology Park, Nano Gostaran Navabegh Fardaye Dashtestan Company, Borazjan, Iran. payamhayati@yahoo.com., Noori F; Student's Research Committee, Faculty of Pharmacy, Kermanshah University of Medical Sciences, Kermanshah, 6714415153, Iran., Mansouri K; Medical Biology Research Center, Health Technology Institute, Kermanshah University of Medical Sciences, Kermanshah, Iran., Moakedi F; Department of Biochemistry and Molecular Biology, School of Medicine, West Virginia University, Morgantown, USA., Janczak J; Institute of Low Temperature and Structure Research Polish Academy of Sciences, P.O. Box 1410, Okolna 2 str., 50-950, Wrocław, Poland., Soltanian Fard MJ; Department of Chemistry, Faculty of Chemical Science, Firoozabad Branch, Islamic Azad University, P.O. Box 74715-117, Firoozabad, Fars, Iran., Fayaz Bakhsh N; Department of Chemistry, Faculty of Chemical Science, Firoozabad Branch, Islamic Azad University, P.O. Box 74715-117, Firoozabad, Fars, Iran. |
| Abstrakt: |
This scenario was designed to investigate the protein corona pattern on the pillar-layer surface of a Cu-based metal-organic framework (MOF) in human plasma. The [Cu(L)(L / )].1.3DMA (MOF-1) {L = 4, 4 / -bipyridine and L / = 5-aminoisophthalic acid}, was synthesized through the sonochemical irradiation approach as well as characterized by various techniques like scanning electron microscopy, Fourier-transform infrared spectroscopy, X-ray powder diffraction and single-crystal X-ray diffraction. The space group was determined to be an orthorhombic space group (Pbam) by single-crystal X-ray diffraction. Single-crystal X-ray analyses on MOF-1 showed that Cu +2 ion was 6-coordinated. Besides, to study and clarify interactions between MOFs and biological milieu, human whole blood plasma was selected as a model. Fluorescence spectroscopy and SDS-PAGE techniques were employed to explore quantitative and qualitative in situ characterization of protein corona as well. Furthermore, cell viability in a cancerous cell lines was evaluated by MTT assay in the presence and absence of the corona. The results from SDS-PAGE illustrated that the most adsorbed quantity among plasma proteins belongs to fibrinogen (α, β and γ chains), and this protein showed the maximum frequency on the MOF-1s surface, so the possible interactions of MOF-1s with fibrinogen also studied using fluorescence spectroscopy and corresponding data were plotted. According to the obtained data from MTT assay, these structures have concentration-dependent toxicity. In brief, based on the obtained data in the current study, the designed MOF can be introduced as a new desirable carrier for drug/gen delivery after further prerequisite assessments. |
