Brazilian Copaifera Species: Antifungal Activity against Clinically Relevant Candida Species, Cellular Target, and In Vivo Toxicity.

Autor: Andrade G; University of Franca, Franca 14404-600, Brazil., Orlando HCS; University of Franca, Franca 14404-600, Brazil., Scorzoni L; School of Pharmaceutical Sciences, São Paulo State University (UNESP), Araraquara 14801-902, Brazil.; Science and Technology Institute of São José dos Campos (ICT), São Paulo State University (UNESP), São José dos Campos 12245-000, Brazil., Pedroso RS; University of Franca, Franca 14404-600, Brazil.; Health Technical School (ESTES), Federal University of Uberlandia, Uberlandia 38400-732, Brazil., Abrão F; University of Franca, Franca 14404-600, Brazil., Carvalho MTM; University of Franca, Franca 14404-600, Brazil., Veneziani RCS; University of Franca, Franca 14404-600, Brazil., Ambrósio SR; University of Franca, Franca 14404-600, Brazil., Bastos JK; Faculty of Pharmaceutical Sciences of Ribeirao Preto, University of Sao Paulo, Ribeirão Preto 14040-903, Brazil., Mendes-Giannini MJS; School of Pharmaceutical Sciences, São Paulo State University (UNESP), Araraquara 14801-902, Brazil., Martins CHG; Institute of Biomedical Sciences (ICBIM), Federal University of Uberlandia, Uberlandia 38400-902, Brazil., Pires RH; University of Franca, Franca 14404-600, Brazil.
Jazyk: angličtina
Zdroj: Journal of fungi (Basel, Switzerland) [J Fungi (Basel)] 2020 Aug 28; Vol. 6 (3). Date of Electronic Publication: 2020 Aug 28.
DOI: 10.3390/jof6030153
Abstrakt: Plants belonging to the genus Copaifera are widely used in Brazil due to their antimicrobial properties, among others. The re-emergence of classic fungal diseases as a consequence of antifungal resistance to available drugs has stimulated the search for plant-based compounds with antifungal activity, especially against Candida . The Candida -infected Caenorhabditis elegans model was used to evaluate the in vitro antifungal potential of Copaifera leaf extracts and trunk oleoresins against Candida species. The Copaifera leaf extracts exhibited good antifungal activity against all Candida species, with MIC values ranging from 5.86 to 93.75 µg/mL. Both the Copaifera paupera and Copaifera reticulata leaf extracts at 46.87 µg/mL inhibited Candida glabrata biofilm formation and showed no toxicity to C. elegans. The survival of C. glabrata -infected nematodes increased at all the tested extract concentrations. Exposure to Copaifera leaf extracts markedly increased C. glabrata cell vacuolization and cell membrane damage. Therefore, Copaifera leaf extracts are potential candidates for the development of new and safe antifungal agents.
Databáze: MEDLINE
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