Engineering drought tolerance in plants through CRISPR/Cas genome editing.

Autor: Joshi RK; Department of Biotechnology, Rama Devi Women's University, Vidya Vihar, Bhubaneswar, Odisha India., Bharat SS; National Key Facility for Crop Gene Resources and Genetic Improvement (NFCRI), Institute of Crop Science, Chinese Academy of Agriculture Sciences (CAAS), Beijing, 100081 China., Mishra R; School of Applied Sciences, Centurion University of Technology and Management, Bhubaneswar, Odisha India.
Jazyk: angličtina
Zdroj: 3 Biotech [3 Biotech] 2020 Sep; Vol. 10 (9), pp. 400. Date of Electronic Publication: 2020 Aug 19.
DOI: 10.1007/s13205-020-02390-3
Abstrakt: Drought stress is primarily responsible for heavy yield losses and productivity in major crops and possesses the greatest threat to the global food security. While conventional and molecular breeding approaches along with genetic engineering techniques have been instrumental in developing drought-tolerant crop varieties, these methods are cumbersome, time consuming and the genetically modified varieties are not widely accepted due to regulatory concerns. Plant breeders are now increasingly centring towards the recently available genome-editing tools for improvement of agriculturally important traits. The advent of multiple sequence-specific nucleases has facilitated precise gene modification towards development of novel climate ready crop variants. Amongst the available genome-editing platforms, the clustered regularly interspaced short palindromic repeat-Cas (CRISPR/Cas) system has emerged as a revolutionary tool for its simplicity, adaptability, flexibility and wide applicability. In this review, we focus on understanding the molecular mechanism of drought response in plants and the application of CRISPR/Cas genome-editing system towards improved tolerance to drought stress.
Competing Interests: Conflict of interestThe authors declare that there is no conflict of interest.
(© King Abdulaziz City for Science and Technology 2020.)
Databáze: MEDLINE
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