Copro-microscopical and immunological diagnosis of cryptosporidiosis in Egyptian buffalo-calves with special reference to their cytokine profiles.

Autor: Aboelsoued D; Department of Parasitology and Animal Diseases, National Research Centre, 33 El Buhouth St., Dokki, Cairo, Egypt., Hendawy SHM; Department of Parasitology and Animal Diseases, National Research Centre, 33 El Buhouth St., Dokki, Cairo, Egypt., Abo-Aziza FAM; Department of Parasitology and Animal Diseases, National Research Centre, 33 El Buhouth St., Dokki, Cairo, Egypt., Abdel Megeed KN; Department of Parasitology and Animal Diseases, National Research Centre, 33 El Buhouth St., Dokki, Cairo, Egypt.
Jazyk: angličtina
Zdroj: Journal of parasitic diseases : official organ of the Indian Society for Parasitology [J Parasit Dis] 2020 Sep; Vol. 44 (3), pp. 654-660. Date of Electronic Publication: 2020 Jul 17.
DOI: 10.1007/s12639-020-01244-2
Abstrakt: Cryptosporidiosis is considered to be one of the most devasting gastrointestinal diseases in calves. The aim of this study was to investigate Cryptosporidium parvum infection ( C. parvum ) in buffalo-calves with both copro-microscopic examination and enzyme linked immunosorbent assay (ELISA) using two C. parvum prepared antigens with regards to their cytokines profile; interferon- γ (IFN-γ), interleukin (IL)-12 and IL-14 to achieve a proper diagnosis. All collected buffalo- calves' fecal samples were examined by modified Ziehl-Neelsen staining technique. ELISA was performed to evaluate the diagnostic accuracy of the two C. parvum prepared antigens; crude whole oocyst (CWO) and crude sonicated oocyst (CSO) in detection of anti- C. parvum IgG in buffalo-calves' sera. As well, concentrations of INF-γ, IL-12 and IL-14 in the buffalo-calves' serum samples were estimated. The results revealed that the overall parasitological incidence of cryptosporidiosis was 40%. However, the serological diagnosis by ELISA assay showed 53.75% and 27.5% when using CWO and CSO antigen, respectively. Also, the diagnostic efficacy parameters of both antigens; CWO and CSO showed a significant high specificity (83.3%) achieved by CSO antigen and a high sensitivity (71.8%) by CWO antigen. The levels of INF-γ, IL-12 and IL-14 were significantly increased in positive Cryptosporidium infected group by both coprological and serological assays followed by the group which was positive for cryptosporidiosis by copro-microscopic examination only. The present study concluded that a combination of coprological and serological examination with reference to the cytokines profile is needed for proper diagnosis of cryptosporidiosis in buffalo-calves.
Competing Interests: Conflicts of interestThe authors declare that they have no conflicts or competing interests.
(© Indian Society for Parasitology 2020.)
Databáze: MEDLINE
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