The Ribbon-Helix-Helix Domain Protein CdrS Regulates the Tubulin Homolog ftsZ2 To Control Cell Division in Archaea.

Autor:	Darnell CL; Biology Department, Duke University, Durham, North Carolina, USA., Zheng J; Department of Molecular and Cellular Biology, Harvard University, Cambridge, Massachusetts, USA., Wilson S; Department of Molecular and Cellular Biology, Harvard University, Cambridge, Massachusetts, USA., Bertoli RM; Biology Department, Duke University, Durham, North Carolina, USA., Bisson-Filho AW; Department of Biology, Rosenstiel Basic Medical Science Research Center, Brandeis University, Waltham, Massachusetts, USA., Garner EC; Department of Molecular and Cellular Biology, Harvard University, Cambridge, Massachusetts, USA., Schmid AK; Biology Department, Duke University, Durham, North Carolina, USA amy.schmid@duke.edu.; Center for Genomics and Computational Biology, Duke University, Durham, North Carolina, USA.
Jazyk:	angličtina
Zdroj:	MBio [mBio] 2020 Aug 11; Vol. 11 (4). Date of Electronic Publication: 2020 Aug 11.
DOI:	10.1128/mBio.01007-20
Abstrakt:	Precise control of the cell cycle is central to the physiology of all cells. In prior work we demonstrated that archaeal cells maintain a constant size; however, the regulatory mechanisms underlying the cell cycle remain unexplored in this domain of life. Here, we use genetics, functional genomics, and quantitative imaging to identify and characterize the novel CdrSL gene regulatory network in a model species of archaea. We demonstrate the central role of these ribbon-helix-helix family transcription factors in the regulation of cell division through specific transcriptional control of the gene encoding FtsZ2, a putative tubulin homolog. Using time-lapse fluorescence microscopy in live cells cultivated in microfluidics devices, we further demonstrate that FtsZ2 is required for cell division but not elongation. The cdrS-ftsZ2 locus is highly conserved throughout the archaeal domain, and the central function of CdrS in regulating cell division is conserved across hypersaline adapted archaea. We propose that the CdrSL-FtsZ2 transcriptional network coordinates cell division timing with cell growth in archaea. IMPORTANCE Healthy cell growth and division are critical for individual organism survival and species long-term viability. However, it remains unknown how cells of the domain Archaea maintain a healthy cell cycle. Understanding the archaeal cell cycle is of paramount evolutionary importance given that an archaeal cell was the host of the endosymbiotic event that gave rise to eukaryotes. Here, we identify and characterize novel molecular players needed for regulating cell division in archaea. These molecules dictate the timing of cell septation but are dispensable for growth between divisions. Timing is accomplished through transcriptional control of the cell division ring. Our results shed light on mechanisms underlying the archaeal cell cycle, which has thus far remained elusive. (Copyright © 2020 Darnell et al.)
Databáze:	MEDLINE
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