[Chromato-mass spectrometric analysis of urine proteins associated with the functions of Toll-receptors in a healthy person under conditions of 17-day isolation.]

Autor:	Zakharova NB; V. Razumovsky State medical University of Saratov Ministry of health of Russia, 410012, Saratov, Russia., Pastyshkova LK; Institute of biomedical problems RAN 123007, Moscow, Russia., Goncharova AG; Institute of biomedical problems RAN 123007, Moscow, Russia., Orlova KD; Institute of biomedical problems RAN 123007, Moscow, Russia., Kashirina DN; Institute of biomedical problems RAN 123007, Moscow, Russia., Goncharov IN; Institute of biomedical problems RAN 123007, Moscow, Russia., Brzhozovsky AG; Institute of biomedical problems RAN 123007, Moscow, Russia., Ponomarev SA; Institute of biomedical problems RAN 123007, Moscow, Russia., Morozova OL; Department of Pathophysiology, Sechenov First Moscow State Medical University (Sechenov University), 119991, Moscow, Russia., Larina IM; Institute of biomedical problems RAN 123007, Moscow, Russia.
Jazyk:	ruština
Zdroj:	Klinicheskaia laboratornaia diagnostika [Klin Lab Diagn] 2020 Jul 20; Vol. 65 (8), pp. 469-473.
DOI:	10.18821/0869-2084-2020-65-8-469-473
Abstrakt:	Under controlled conditions of 17-day isolation (Sirius-17 experiment), the protein composition of urine was studied in 6 healthy test volunteers-3 women and 3 men. Collection of samples in the form of a second freely separated morning urine fraction was carried out in the background (seven days before the experiment), as well as 1 day after the end of exposure. Chromatographic-mass-spectrometric semi-quantitative analysis of the protein composition of samples was performed on a system consisting of an Agilent 1100 chromatograph and an LTQ-FT Ultra hybrid mass spectrometer using bioinformatics resources UniProtKB, GeneOntology. An asymptomatic change in the immune defense system of kidney tissue after isolation in a closed hermetic object is associated with a change in the content of 7 proteins that provide functional activity of the TLR tubules of the kidneys - FcRIII, MUC1, Galectin-3, Ficolin-2, APOA1, FLNA, FCGR3A and Clusterin. These proteins are found to be useful biomarkers in the study of physiology and kidney diseases. They can be attributed to candidates for protein markers of the initial stages of impaired recognition by the epithelium of renal tubules of bacteria with known pathogenic potential. Competing Interests: The authors declare no conflict of interest.
Databáze:	MEDLINE
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