The wheat Sr22, Sr33, Sr35 and Sr45 genes confer resistance against stem rust in barley.

Autor: Hatta MAM; John Innes Centre, Norwich Research Park, Norwich, UK.; Department of Agriculture Technology, Faculty of Agriculture, Universiti Putra Malaysia, Serdang, Malaysia., Arora S; John Innes Centre, Norwich Research Park, Norwich, UK., Ghosh S; John Innes Centre, Norwich Research Park, Norwich, UK., Matny O; Department of Plant Pathology, Stakman Borlaug Center for Sustainable Plant Health, University of Minnesota, St. Paul, MN, USA., Smedley MA; John Innes Centre, Norwich Research Park, Norwich, UK., Yu G; John Innes Centre, Norwich Research Park, Norwich, UK., Chakraborty S; Commonwealth Scientific and Industrial Research Organization (CSIRO), Agriculture and Food, Canberra, ACT, Australia., Bhatt D; Commonwealth Scientific and Industrial Research Organization (CSIRO), Agriculture and Food, Canberra, ACT, Australia., Xia X; Commonwealth Scientific and Industrial Research Organization (CSIRO), Agriculture and Food, Canberra, ACT, Australia., Steuernagel B; John Innes Centre, Norwich Research Park, Norwich, UK., Richardson T; Commonwealth Scientific and Industrial Research Organization (CSIRO), Agriculture and Food, Canberra, ACT, Australia., Mago R; Commonwealth Scientific and Industrial Research Organization (CSIRO), Agriculture and Food, Canberra, ACT, Australia., Lagudah ES; Commonwealth Scientific and Industrial Research Organization (CSIRO), Agriculture and Food, Canberra, ACT, Australia., Patron NJ; The Earlham Institute, Norwich Research Park, Norwich, UK., Ayliffe M; Commonwealth Scientific and Industrial Research Organization (CSIRO), Agriculture and Food, Canberra, ACT, Australia., Rouse MN; Department of Plant Pathology, Stakman Borlaug Center for Sustainable Plant Health, University of Minnesota, St. Paul, MN, USA.; USDA-ARS Cereal Disease Laboratory, St. Paul, MN, USA., Harwood WA; John Innes Centre, Norwich Research Park, Norwich, UK., Periyannan S; Commonwealth Scientific and Industrial Research Organization (CSIRO), Agriculture and Food, Canberra, ACT, Australia., Steffenson BJ; Department of Plant Pathology, Stakman Borlaug Center for Sustainable Plant Health, University of Minnesota, St. Paul, MN, USA., Wulff BBH; John Innes Centre, Norwich Research Park, Norwich, UK.
Jazyk: angličtina
Zdroj: Plant biotechnology journal [Plant Biotechnol J] 2021 Feb; Vol. 19 (2), pp. 273-284. Date of Electronic Publication: 2020 Sep 06.
DOI: 10.1111/pbi.13460
Abstrakt: In the last 20 years, stem rust caused by the fungus Puccinia graminis f. sp. tritici (Pgt), has re-emerged as a major threat to wheat and barley production in Africa and Europe. In contrast to wheat with 60 designated stem rust (Sr) resistance genes, barley's genetic variation for stem rust resistance is very narrow with only ten resistance genes genetically identified. Of these, only one complex locus consisting of three genes is effective against TTKSK, a widely virulent Pgt race of the Ug99 tribe which emerged in Uganda in 1999 and has since spread to much of East Africa and parts of the Middle East. The objective of this study was to assess the functionality, in barley, of cloned wheat Sr genes effective against race TTKSK. Sr22, Sr33, Sr35 and Sr45 were transformed into barley cv. Golden Promise using Agrobacterium-mediated transformation. All four genes were found to confer effective stem rust resistance. The barley transgenics remained susceptible to the barley leaf rust pathogen Puccinia hordei, indicating that the resistance conferred by these wheat Sr genes was specific for Pgt. Furthermore, these transgenic plants did not display significant adverse agronomic effects in the absence of disease. Cloned Sr genes from wheat are therefore a potential source of resistance against wheat stem rust in barley.
(© 2020 The Authors. Plant Biotechnology Journal published by Society for Experimental Biology and The Association of Applied Biologists and John Wiley & Sons Ltd.)
Databáze: MEDLINE
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