| Autor: |
Machado VS; Laboratório de Pesquisa em Microbiologia Oral - LAPEMICRO, UFSM, Santa Maria, Brazil., Verdi CM; Laboratório de Pesquisa em Microbiologia Oral - LAPEMICRO, UFSM, Santa Maria, Brazil., Somacal S; Núcleo Integrado de Desenvolvimento em Análises Laboratoriais - NIDAL, UFSM, Santa Maria, RS, Brasil., Rossi GG; Laboratório de Micobacteriologia - LABIMYCO, UFSM, Santa Maria, Brazil., Machado ML; Departamento de Bioquímica Toxicológica, Universidade Federal de Santa Maria (UFSM), Santa Maria, RS, Brasil., Klein B; Núcleo Integrado de Desenvolvimento em Análises Laboratoriais - NIDAL, UFSM, Santa Maria, RS, Brasil., Roos VC; Laboratório de Pesquisa em Microbiologia Oral - LAPEMICRO, UFSM, Santa Maria, Brazil., Urquhart CG; Laboratório de Pesquisa em Microbiologia Oral - LAPEMICRO, UFSM, Santa Maria, Brazil., Dalcol II; Departamento de Química, UFSM, Santa Maria, Brazil., Sagrillo MR; Laboratório de Cultivo Celular, Programa de Pós-graduação em Nanociências - UFN, Santa Maria, Brazil., Machado AK; Laboratório de Cultivo Celular, Programa de Pós-graduação em Nanociências - UFN, Santa Maria, Brazil., Campos MM; Laboratório de Micobacteriologia - LABIMYCO, UFSM, Santa Maria, Brazil., Wagner R; Núcleo Integrado de Desenvolvimento em Análises Laboratoriais - NIDAL, UFSM, Santa Maria, RS, Brasil., Santos RCV; Laboratório de Pesquisa em Microbiologia Oral - LAPEMICRO, UFSM, Santa Maria, Brazil. |
| Abstrakt: |
Achyrocline flaccida (Weinm) DC. essential oil was obtained by hydrodistillation of wild flowers from southern Brazil. We explored, for the first time, the phytochemical composition, toxicity, resistance to oxidative stress in Caenorhabditis elegans , and antimycobacterial activities of A. flaccida essential oil. Twenty-four compounds were identified by GC-FID-MS and the major constituents identified were α-pinene (41.10%) and caryophyllene (30.52%). The essential oil showed no signs of genotoxicity in the comet assay and presented relevant antioxidant capacity since it prevented ROS production in the C. elegans model. Furthermore, the minimal inhibitory concentrations (MIC) results showed that M. abscessus , M. massiliense , and M. fortuitum had their growth inhibited by A. flaccida essential oil. Therefore, the essential oil of this plant is a promising alternative in the search for new compounds capable of decreasing oxidative stress and treating mycobacteriosis. |
