rkDNA-graphene oxide as a simple probe for the rapid detection of miRNA21.

Autor: Choi MH; Department of Chemistry, Jeonbuk National University, Jeonju 54896, South Korea., Ravi Kumara GS; Department of Chemistry, Jeonbuk National University, Jeonju 54896, South Korea., Seo YJ; Department of Chemistry, Jeonbuk National University, Jeonju 54896, South Korea. Electronic address: yseo@jbnu.ac.kr.
Jazyk: angličtina
Zdroj: Bioorganic & medicinal chemistry letters [Bioorg Med Chem Lett] 2020 Sep 01; Vol. 30 (17), pp. 127398. Date of Electronic Publication: 2020 Jul 15.
DOI: 10.1016/j.bmcl.2020.127398
Abstrakt: In this study we developed a novel diagnostic tool for the detection of miRNA21, based on the fluorescent nucleotide morpholine naphthalimide deoxyuridine (dUrkTP). We incorporated dUrkTP into DNA through primer extension to obtain rkDNA displaying high fluorescence. We then used lambda exonuclease, a specific nuclease for 3́-monophosphate-containing DNA, to separate rkDNA from its complementary sequence. The fluorescence of the free rkDNA was quenched dramatically upon interacting with graphene oxide (GO). Our rkDNA-GO fluorescence probing system exhibited high sensitivity and selectivity for the detection of miRNA21. This inexpensive probing system, employing simple primer extension and exonuclease degradation, required only 30 min to detect its target miRNA. This strategy appears suitable for the detection of diverse types of miRNA.
Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2020 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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